Li Qian, Fu Lei, Wu Daoyuan, Wang Jufeng
Department of Oncology, The Affiliated Tumor Hospital of Zhengzhou University, Henan Cancer Hospital, 127 Dongming Road, Jinshui District, Zhengzhou, Henan, China.
Department of Oncology, Henan Provincial People's Hospital, Zhengzhou, Henan, China.
Biochem Genet. 2023 Apr;61(2):669-686. doi: 10.1007/s10528-022-10276-7. Epub 2022 Sep 12.
Neuronal regeneration related protein (NREP), also known as P311, has been reported to participate in multiple biological processes. The detection of tumor biomarker favored a non-invasive early entry for cancer diagnosis and disease monitoring to prevent its worsening symptoms. This study is intended to investigate the clinical roles of NREP in gastric cancer (GC) and its effect on gastric cancer cell proliferation and angiogenesis. Our results demonstrated that NREP was typically upregulated in GC tissues compared with normal control. The Kaplan-Meier analysis showed correlations between increased NREP level and poor survival, indicating the prognostic value of NREP in GC patients. The expression levels of NREP varied by races, clinical T stages, and histologic grades. NREP expression was associated with tumor-associated immune infiltration. The NREP expression was powerfully associated with clinical characteristics of GC patients, in particular, with T stage and histologic grade. Gene ontology and KEGG signaling analysis indicated that NREP-related genes were predominantly enriched in various pathways. Additionally, knockdown of NREP inhibited human gastric adenocarcinoma cell proliferation and angiogenesis. Collectively, our results suggested that NREP may be an excellent biomarker for the clinical diagnosis, prognosis, and therapy of GC.
神经元再生相关蛋白（NREP），也称为P311，据报道参与多种生物学过程。肿瘤生物标志物的检测有利于癌症诊断和疾病监测的非侵入性早期介入，以防止症状恶化。本研究旨在探讨NREP在胃癌（GC）中的临床作用及其对胃癌细胞增殖和血管生成的影响。我们的结果表明，与正常对照相比，NREP在GC组织中通常上调。Kaplan-Meier分析显示NREP水平升高与不良生存之间存在相关性，表明NREP在GC患者中的预后价值。NREP的表达水平因种族、临床T分期和组织学分级而异。NREP表达与肿瘤相关免疫浸润有关。NREP表达与GC患者的临床特征密切相关，特别是与T分期和组织学分级有关。基因本体和KEGG信号分析表明，NREP相关基因主要富集于各种途径。此外，敲低NREP可抑制人胃腺癌细胞的增殖和血管生成。总的来说，我们的结果表明，NREP可能是GC临床诊断、预后和治疗的优秀生物标志物。
