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比较评价 Re 529-bp 序列和 B1 基因在土耳其代尼兹利水样中通过 PCR 检测弓形虫的应用。

Comparative Evaluation of Re 529-Bp Sequence and B1 Gene in the Detection of Toxoplasma gondii Through PCR in Water Samples of Denizli, Turkey.

机构信息

Department of Biology, Faculty of Arts and Science, Bolu Abant İzzet Baysal University, 14030, Bolu, Turkey.

Department of Biology, Faculty of Arts and Science, Pamukkale University, 20180, Denizli, Turkey.

出版信息

Acta Parasitol. 2022 Mar;67(1):555-559. doi: 10.1007/s11686-021-00494-1. Epub 2021 Nov 24.

DOI:10.1007/s11686-021-00494-1
PMID:34817741
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8611623/
Abstract

PURPOSE

While Toxoplasma gondii (T. gondii) infection is asymptomatic in immunocompetent individuals, it is a life-threatening protozoan in immunocompromised individuals. Its water-borne transmission to humans poses a serious public health concern. Polymerase Chain Reaction (PCR) has a considerable potential for the sensitive and specific detection of T. gondii oocysts in waters.

METHODS

Comparative evaluation of RE 529-bp sequence and B1 gene to detect T. gondii tachyzoites and oocysts via PCR in agricultural irrigation water taken from downtown Denizli, Turkey and water samples collected from neighborhood fountains was performed for the first time in Turkish context.

RESULTS

Based on real-time PCR targeting the B1 genetic markers and RE 529-bp sequence, T. gondii DNA was identified in 6 (16.7%) out of 48 samples collected from agricultural irrigation water. Besides, our PCR analysis did not establish any presence of T. gondii in drinking water samples.

CONCLUSION

T. gondii showed lower sensitivity in B1-based PCR than in PCR targeting RE 529-bp sequence.

摘要

目的

虽然刚地弓形虫（Toxoplasma gondii，T. gondii）在免疫功能正常的个体中感染通常无症状，但它对于免疫功能低下的个体来说是一种危及生命的原生动物。其通过水传播给人类，引起了严重的公共卫生关注。聚合酶链反应（PCR）具有很高的潜力，可用于敏感且特异性地检测水中的 T. gondii 卵囊。

方法

首次在土耳其背景下，比较评估了 RE 529-bp 序列和 B1 基因，以通过 PCR 检测来自土耳其代尼兹利市中心的农业灌溉水中的刚地弓形虫速殖子和卵囊，以及从附近喷泉采集的水样。

结果

基于针对 B1 遗传标记和 RE 529-bp 序列的实时 PCR，从 48 个采集自农业灌溉水的样本中，有 6 个（16.7%）样本中检测到了 T. gondii DNA。此外，我们的 PCR 分析并未在饮用水样本中发现任何 T. gondii 的存在。

结论

B1 基因为基础的 PCR 检测方法比针对 RE 529-bp 序列的 PCR 检测方法显示出更低的敏感性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0aec/8611623/0288f3c08219/11686_2021_494_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0aec/8611623/e55112c07045/11686_2021_494_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0aec/8611623/0288f3c08219/11686_2021_494_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0aec/8611623/e55112c07045/11686_2021_494_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0aec/8611623/0288f3c08219/11686_2021_494_Fig2_HTML.jpg

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