Department of Radiology, Hainan General Hospital, Affiliated Hainan Hospital of Hainan Medical College, No.19 Xiuhua Road, Xiuying District, Haikou, 570311, Hainan, China.
Department of Radiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.
Funct Integr Genomics. 2022 Feb;22(1):55-64. doi: 10.1007/s10142-021-00812-w. Epub 2021 Nov 24.
Long intergenic non-coding RNA 01,087 (LINC01087) has been concerned as an oncogene in breast cancer, while its mechanism in glioma has been little surveyed. Thus, we searched the prognostic value and functional action of LINC01087 in glioma. Glioma patients after preoperative MRI diagnosis were enrolled, and LINC01087, microRNA (miR)-1277-5p, and alkaline ceramidase 3 (ACER3) levels were tested in glioma cancer tissue. The correlation between LINC01087 expression and the survival of patients were analyzed. LINC01087, miR-1277-5p, and ACER3 levels in U251 cells were altered via transfection, and cell malignant phenotypes were monitored. The relationship between miR-1277-5p and LINC01087 or ACER3 was detected. The LINC01087 and ACER3 expression was in up-regulation and the miR-1277-5p expression was in down-regulation in clinical glioma samples. High expression of LINC01087 was associated with poor prognosis of glioma patients with preoperative MRI. LINC01087 silencing restrained tumor malignancy in glioma cells. Mechanistically, LINC01087 directly interacted with miR-1277-5p. ACER3 was a known target of miR-1277-5p. Moreover, rescue assays reveal that miR-1277-5p overexpression (or ACER3 overexpression) reversed the effects of LINC01087 upregulation (or miR-1277-5p upregulation) on glioma cells. LINC01087 has prognostic significance in glioma and silencing LINC01087 deters glioma development through elevating miR-1277-5p to reduce ACER3 expression.
长链非编码 RNA 01087（LINC01087）已被认为是乳腺癌的致癌基因，但其在神经胶质瘤中的作用机制尚未得到广泛研究。因此，我们研究了 LINC01087 在神经胶质瘤中的预后价值和功能作用。招募了术前 MRI 诊断后的神经胶质瘤患者，并检测了神经胶质瘤组织中的 LINC01087、微小 RNA（miR）-1277-5p 和碱性角质酶 3（ACER3）水平。分析了 LINC01087 表达与患者生存的相关性。通过转染改变 U251 细胞中的 LINC01087、miR-1277-5p 和 ACER3 水平，并监测细胞恶性表型。检测了 miR-1277-5p 与 LINC01087 或 ACER3 之间的关系。临床神经胶质瘤样本中 LINC01087 和 ACER3 的表达上调，miR-1277-5p 的表达下调。LINC01087 高表达与术前 MRI 神经胶质瘤患者的不良预后相关。LINC01087 沉默抑制神经胶质瘤细胞的肿瘤恶性。机制上，LINC01087 与 miR-1277-5p 直接相互作用。ACER3 是 miR-1277-5p 的已知靶标。此外，挽救实验表明，miR-1277-5p 过表达（或 ACER3 过表达）逆转了 LINC01087 上调（或 miR-1277-5p 上调）对神经胶质瘤细胞的影响。LINC01087 在神经胶质瘤中具有预后意义，沉默 LINC01087 通过提高 miR-1277-5p 来降低 ACER3 表达来阻止神经胶质瘤的发展。
