长链非编码RNA H19对大鼠坐骨神经损伤后神经退变与再生的影响
Effect of lncRNA H19 on nerve degeneration and regeneration after sciatic nerve injury in rats.
作者信息
Li Yuting, Cai Min, Feng Yumei, Yung Bryant, Wang Yi, Gao Nannan, Xu Xi, Zhang Huanhuan, Huang Huiwei, Yao Dengbing
机构信息
School of Life Sciences, Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, P. R. China.
Diagnostic laboratory, Medical School of Nantong University, Nantong, P. R. China.
出版信息
Dev Neurobiol. 2022 Jan;82(1):98-111. doi: 10.1002/dneu.22861. Epub 2021 Dec 10.
Hundreds of millions of people worldwide suffer from peripheral nerve damage resulting from car accidents, falls, industrial accidents, residential accidents, and wars. The purpose of our study was to further investigate the effects of Wallerian degeneration (WD) after rat sciatic nerve injury and to screen for critical long noncoding RNAs (lncRNAs) in WD. We found H19 to be essential for nerve degeneration and regeneration and to be highly expressed in the sciatic nerves of rats with WD. lncRNA H19 potentially impaired the recovery of sciatic nerve function in rats. H19 was mainly localized in the cytoplasm of Schwann cells (SCs) and promoted their migration. H19 promoted the apoptosis of dorsal root ganglion (DRG) neurons and slowed the growth of DRG axons. The lncRNA H19 may play a role in WD through the Wnt/β-catenin signaling pathway and is coexpressed with a variety of crucial mRNAs during WD. These data provide further insight into the molecular mechanisms of WD.
全球数亿人因车祸、跌倒、工业事故、家庭事故和战争而遭受周围神经损伤。我们研究的目的是进一步研究大鼠坐骨神经损伤后沃勒变性（WD）的影响，并筛选WD中的关键长链非编码RNA（lncRNA）。我们发现H19对神经变性和再生至关重要，并且在WD大鼠的坐骨神经中高表达。lncRNA H19可能会损害大鼠坐骨神经功能的恢复。H19主要定位于雪旺细胞（SCs）的细胞质中，并促进其迁移。H19促进背根神经节（DRG）神经元的凋亡，并减缓DRG轴突的生长。lncRNA H19可能通过Wnt/β-连环蛋白信号通路在WD中发挥作用，并且在WD期间与多种关键mRNA共表达。这些数据为WD的分子机制提供了进一步的见解。
Zotero 插件