Camilleri-Robles Carlos, Amador Raziel, Tiebe Marcel, Teleman Aurelio A, Serras Florenci, Guigó Roderic, Corominas Montserrat
Departament de Genètica, Microbiologia i Estadística, Facultat de Biologia and Institut de Biomedicina (IBUB), Universitat de Barcelona, Barcelona, Catalonia, Spain.
Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology (BIST), Barcelona, Catalonia, Spain.
NAR Genom Bioinform. 2024 Aug 16;6(3):lqae091. doi: 10.1093/nargab/lqae091. eCollection 2024 Sep.
The discovery of functional long non-coding RNAs (lncRNAs) changed their initial concept as transcriptional noise. LncRNAs have been identified as regulators of multiple biological processes, including chromatin structure, gene expression, splicing, mRNA degradation, and translation. However, functional studies of lncRNAs are hindered by the usual lack of phenotypes upon deletion or inhibition. Here, we used imaginal discs as a model system to identify lncRNAs involved in development and regeneration. We examined a subset of lncRNAs expressed in the wing, leg, and eye disc development. Additionally, we analyzed transcriptomic data from regenerating wing discs to profile the expression pattern of lncRNAs during tissue repair. We focused on the lncRNA , which is upregulated during regeneration. We generated mutant flies that developed normally but showed impaired wing regeneration upon cell death induction. The ability of these mutants to regenerate was restored by the ectopic expression of . Furthermore, we found that the lncRNA has a high degree of sequence similarity with and can partially compensate for its function during regeneration in the absence of . Our findings point to a potential role of the lncRNA in during the response to damage in the wing imaginal disc.
功能性长链非编码RNA（lncRNA）的发现改变了它们最初作为转录噪音的概念。lncRNA已被确定为多种生物学过程的调节因子，包括染色质结构、基因表达、剪接、mRNA降解和翻译。然而，lncRNA的功能研究受到通常在缺失或抑制时缺乏表型的阻碍。在这里，我们使用成虫盘作为模型系统来鉴定参与发育和再生的lncRNA。我们检查了在翅、腿和眼盘发育中表达的lncRNA子集。此外，我们分析了来自再生翅盘的转录组数据，以描绘lncRNA在组织修复过程中的表达模式。我们重点研究了在再生过程中上调的lncRNA。我们生成了正常发育但在诱导细胞死亡后翅再生受损的突变果蝇。这些突变体的再生能力通过异位表达得以恢复。此外，我们发现lncRNA与具有高度的序列相似性，并且在不存在的情况下在再生过程中可以部分补偿其功能。我们的发现表明lncRNA在翅成虫盘损伤反应中的潜在作用。
