Department of Chemistry and Physics, Research Centre CIAIMBITAL, University of Almería, Ctra. Sacramento, s/n, 04120 Almería, Spain.
Department of Engineering, Research Centre CIAIMBITAL, University of Almería, Ctra. Sacramento, s/n, 04120 Almería, Spain.
Food Chem. 2022 Mar 15;372:131263. doi: 10.1016/j.foodchem.2021.131263. Epub 2021 Sep 29.
A combination of FTIR- and NMR-based metabolomics approaches coupled to multivariate data analysis techniques was used for the first time to characterize and explore the metabolic changes along the ripening of two Spanish melon landraces (Jimbee N and Jimbee XL). Furthermore, the fatty acids profile and the antioxidant capacity were investigated by GC-FID and ORAC method, respectively. Despite FTIR was able to identify changes in the sugars content between fruits of different maturity stages, it failed to discriminate between both varieties, being exceeded by NMR in both situations. Increased fruit maturation led to an increase of sucrose, fatty acids and β-carotene, accompanied by a decrease of glucose, fructose, citrate, amino acids, and polyphenols (which were positively correlated with fruits antioxidant activity).
首次采用基于傅里叶变换红外光谱（FTIR）和核磁共振（NMR）的代谢组学方法结合多元数据分析技术，对两个西班牙瓜类地方品种（Jimbee N 和 Jimbee XL）的成熟过程中的代谢变化进行了特征描述和探索。此外，还分别通过气相色谱-火焰离子化检测（GC-FID）和抗氧化能力测定（ORAC）方法研究了脂肪酸组成和抗氧化能力。尽管 FTIR 能够识别不同成熟阶段果实中糖含量的变化，但它无法区分这两个品种，在这两种情况下均被 NMR 超越。随着果实成熟度的增加，蔗糖、脂肪酸和β-胡萝卜素的含量增加，而葡萄糖、果糖、柠檬酸、氨基酸和多酚的含量减少（这些物质与果实的抗氧化活性呈正相关）。
