Programa de Pós-Graduação em Recursos Naturais, Universidade Estadual de Mato Grosso do Sul (UEMS), P.O. Box 351, Dourados, MS CEP 79804-970, Brazil.
Instituto de Ciências Biomédicas, Universidade de São Paulo (USP), Av. Prof. Lineu Prestes, 1374, Butantã, 05508-900 São Paulo, SP, Brazil.
Sci Total Environ. 2022 Jan 20;805:150348. doi: 10.1016/j.scitotenv.2021.150348. Epub 2021 Sep 16.
A new highly sensitive, selective, and inexpensive electrochemical method has been developed for simultaneously detecting diethylstilbestrol (DES) and 17β-estradiol (E2) in environmental samples (groundwater and lake water) using a graphite sensor modified by cerium oxide nanoparticles (CPE-CeO NPs). The developed sensor and the materials used in its preparation were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The ab initio simulation was used to evaluate the adsorption energies between both DES and E2 with the surface of the sensor. The peak current of oxidation of both hormones showed two regions of linearity. The region of greatest sensitivity was observed for the linear range of 10 nM-100 nM. The detection and quantification limits for this concentration range were 0.8/2.6 nM and 1.3/4.3 nM for DES and E2, respectively. The analytical performance of the developed method showed high sensitivity, precision, repeatability, reproducibility, and selectivity. The CPE-CeO NPs sensor was successfully applied to simultaneously detect DES and E2 in real samples with recovery levels above 98%.
一种新的高灵敏度、选择性和廉价的电化学方法已经开发出来，用于使用氧化铈纳米粒子（CPE-CeO NPs）修饰的石墨传感器同时检测环境样品（地下水和湖水）中的己烯雌酚（DES）和 17β-雌二醇（E2）。使用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和 X 射线衍射（XRD）对开发的传感器和其制备中使用的材料进行了表征。从头算模拟用于评估两种激素与传感器表面之间的吸附能。两种激素的氧化峰电流显示出两个线性区域。在 10 nM-100 nM 的线性范围内观察到最大灵敏度区域。对于该浓度范围，检测限和定量限分别为 0.8/2.6 nM 和 1.3/4.3 nM。所开发方法的分析性能表现出高灵敏度、精度、重复性、再现性和选择性。CPE-CeO NPs 传感器成功地应用于实际样品中同时检测 DES 和 E2，回收率高于 98%。
