Lopes Claudio Sabbatini Capella, Silva Francisco Walison Lima, Fernandes Juliana Dos Santos, Fernandes Julia Oliveira, Ferreira João H A, Brandão Felipe Zandonadi, Santelli Ricardo Erthal, Canevari Thiago C, Cincotto Fernando Henrique
Departamento de Química Analítica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-853, Brazil.
LabNaHm: Multifunctional Hybrid Nanomaterials Laboratory Engineering School, Mackenzie Presbyterian University, São Paulo 01302-907, SP, Brazil.
ACS Omega. 2024 Oct 31;9(48):47729-47738. doi: 10.1021/acsomega.4c07861. eCollection 2024 Dec 3.
One of the main challenges in animal breeding systems is determining estradiol (E2) in livestock samples as simple and minimally invasive as possible, Thus, a nonenzymatic biosensor screen-printed electrode (SPE) was developed by modifying nanohybrid palladium nanoparticles (PdNPs), and carbon dots anchored on a nanosilica particle (PdNPs/C.dots/SiO), denominated SPE/PdNPs/C.dots/SiO, and successfully tested for the direct detection of estradiol in livestock samples. PdNPs were directly obtained by a one-step synthesis through carbon dot reduction. Hybrid nanomaterials were characterized by atomic force microscopy, high-resolution transmission electron microscopy, and electrochemical impedance. The combination of PdNPs with C.dots resulted in a nonenzymatic biosensor supported on a screen-printed platform with superior electrocatalytic properties regarding the oxidation of E2 when compared to unmodified sensors. Modifications in the working electrode resulted in high sensitivity toward E2 determination within a linear range from 0.005 to 14.0 μmol L with a limit of detection of 1.0 nmol L. The recovery rate of E2 in bovine serum samples and urine samples ranged from 92 to 106%. Interference studies showed that peak current variation (Δ ) among all interferents evaluated and E2 did not exceed ±2%. The newly developed sensor stands out not only for its high sensitivity but also for its quick and simple way of production while also being disposable after analysis, providing a simple, sensitive, and practical approach for the determination of reproductive hormones in livestock.
动物育种系统的主要挑战之一是尽可能简单且微创地测定家畜样本中的雌二醇（E2）。因此，通过修饰纳米杂化钯纳米颗粒（PdNPs）和锚定在纳米二氧化硅颗粒上的碳点（PdNPs/C.dots/SiO）开发了一种非酶生物传感器丝网印刷电极（SPE），命名为SPE/PdNPs/C.dots/SiO，并成功用于直接检测家畜样本中的雌二醇。PdNPs通过碳点还原一步合成直接获得。通过原子力显微镜、高分辨率透射电子显微镜和电化学阻抗对杂化纳米材料进行了表征。与未修饰的传感器相比，PdNPs与C.dots的组合产生了一种支撑在丝网印刷平台上的非酶生物传感器，对E2的氧化具有优异的电催化性能。工作电极的修饰导致在0.005至14.0 μmol L的线性范围内对E2测定具有高灵敏度，检测限为1.0 nmol L。牛血清样本和尿液样本中E2的回收率在92%至106%之间。干扰研究表明，所有评估的干扰物与E2之间的峰值电流变化（Δ）不超过±2%。新开发的传感器不仅因其高灵敏度脱颖而出，还因其快速简单的生产方式以及分析后可一次性使用而备受瞩目，为家畜生殖激素的测定提供了一种简单、灵敏且实用的方法。
