Roblin S, Nicollier M, Rémy-Martin J P, Adessi G L
Unité de Recherche de Biochimie Hormonale et des Régulations INSERM U 198, Besançon, France.
Steroids. 1986 Nov-Dec;48(5-6):369-79. doi: 10.1016/0039-128x(86)90023-1.
Lipid content and steroid sulfatase activities were determined in liver and uterus microsomes of non-pregnant guinea pigs. The results were compared with values obtained in pregnant and cortisol-treated animals. Steroid sulfatase activities were always higher in pregnant animals, and we supposed that the increase in circulating cortisol in pregnant guinea pigs before parturition has an influence on the membrane-bound sulfatase activities. Sulfatase activities were identical in cortisol-treated and untreated non-pregnant females, although cortisol induced changes in microsomal lipid composition. These results lead us to three conclusions: in intact female guinea pigs, cortisol induces variations in the lipid content of uterus and liver microsomes, especially in the cholesteryl sulfate to phospholipid ratios; the variations of the lipid composition in pregnant animals do not appear to be cortisol-dependent; membrane-bound steroid sulfatase activities are not directly influenced by the lipid composition of microsomes.
测定了未怀孕豚鼠肝脏和子宫微粒体中的脂质含量及类固醇硫酸酯酶活性。将结果与怀孕动物和皮质醇处理动物的值进行了比较。怀孕动物的类固醇硫酸酯酶活性总是更高，我们推测分娩前怀孕豚鼠循环皮质醇的增加对膜结合硫酸酯酶活性有影响。尽管皮质醇会引起微粒体脂质组成的变化，但皮质醇处理和未处理的未怀孕雌性动物的硫酸酯酶活性相同。这些结果使我们得出三个结论：在完整的雌性豚鼠中，皮质醇会引起子宫和肝脏微粒体脂质含量的变化，尤其是硫酸胆固醇酯与磷脂的比例；怀孕动物脂质组成的变化似乎不依赖于皮质醇；膜结合类固醇硫酸酯酶活性不受微粒体脂质组成的直接影响。
