Prost O, Ottignon Y, Rémy-Martin A, Vuitton D, Miguet J P, Adessi G L
Steroids. 1984 Feb;43(2):189-99. doi: 10.1016/0039-128x(84)90037-0.
Estrone and dehydroepiandrosterone (DHEA) sulfatases were studied in livers of normal and cirrhotic men. Their Km were 3.2 microM and 1.2 microM respectively. The microsomal sulfatases were solubilized by Miranol H2M and ultrasound. After gel filtration, the soluble material gave a single peak of activity for both substrates with a molecular weight of approximately 330,000. In terms of pmol of product.min-1 per mg of fresh tissue, the mean (+/- SD) values of estrone and DHEA sulfatase activities were lower in cirrhotic livers [(n = 7) (4.09 +/- 2.90 and 0.38 +/- 0.20)] than in normal livers [(n = 13)(8.29 +/- 4.00 and 0.69 +/- 0.20)]. The differences were statistically significant : p less than 0.03 for estrone sulfatase and p less than 0.01 for DHEA sulfatase. In cirrhotic men, the mean level of plasma estrone is increased whereas that of estrone sulfate is decreased. The variations may be related to the decrease of serum albumin in cirrhotic subjects.
对正常男性和肝硬化男性的肝脏中的雌酮硫酸酯酶和脱氢表雄酮（DHEA）硫酸酯酶进行了研究。它们的米氏常数（Km）分别为3.2微摩尔和1.2微摩尔。微粒体硫酸酯酶用米那诺尔H2M和超声进行溶解。经过凝胶过滤后，可溶性物质对两种底物均给出一个单一的活性峰，分子量约为330,000。以每毫克新鲜组织每分钟产生的产物皮摩尔数计算，肝硬化肝脏中雌酮和DHEA硫酸酯酶活性的平均值（±标准差）[(n = 7)(4.09 ± 2.90和0.38 ± 0.20)]低于正常肝脏[(n = 13)(8.29 ± 4.00和0.69 ± 0.20)]。差异具有统计学意义：雌酮硫酸酯酶 p < 0.03，DHEA硫酸酯酶 p < 0.01。在肝硬化男性中，血浆雌酮水平升高而硫酸雌酮水平降低。这些变化可能与肝硬化患者血清白蛋白的减少有关。
