Diversity of spp. Associated with Wheat Node and Grain in Representative Sites Across the Western Canadian Prairies.

Fusarium head blight (FHB) and Fusarium crown and root rot (FCRR) are major wheat diseases. Populations of FHB and FCRR pathogens are highly dynamic, and shifts in these populations in different regions is reported. Analyzing fungal populations associated with wheat node and grain tissues collected from different regions can provide useful information and predict diseases that might affect subsequent crops and effective disease management practices. In this study, wheat node and grain samples were collected from four representative sites across the western Canadian prairies in the 2018 growing season to characterize the major spp. and other mycobiota associated with wheat in these regions. In total, 994 fungal isolates were recovered, and based on culture and molecular diagnostic methods, three genera constituted over 90% of all fungal isolates, namely (39.6%), (27.8%), and (23.9%). A quantitative PCR (qPCR) diagnostic toolkit was developed to quantify the most frequently isolated spp. in infected wheat tissues: , , , and . This qPCR specificity was validated in silico, in vitro, and in planta and proved specific to the target species. The qPCR results showed that was not detected frequently from wheat node and grain samples collected from four locations in this study. was the most abundant species in grain samples in all tested locations. However, in node samples, (Beaverlodge and Scott) and (Lacombe and Lethbridge) were the most abundant species. Trichothecene genotyping showed that the 3ADON is the most dominant trichothecene genotype (68%), followed by type-A trichothecenes (29.5%), whereas the 15ADON trichothecene genotype was least dominant (2.5%) and the NIV genotype was not detected. Moreover, a total of 129 translation elongation factor 1-alpha () sequences from nine spp. were compared at the haplotype level to evaluate genetic variability and distribution. and exhibited higher diversity as reflected by higher number of haplotypes present in these two species compared with the rest.