Wang Yufan, Hu Yanling, Gong Fangyi, Jin Yarong, Xia Yingjie, He Yu, Jiang Yun, Zhou Qiang, He Jingshu, Feng Lihua, Chen Guoyue, Zheng Youliang, Liu Dengcai, Huang Lin, Wu Bihua
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Plant Dis. 2022 Apr;106(4):1278-1285. doi: 10.1094/PDIS-09-21-1946-RE. Epub 2022 Mar 30.
Stripe rust, caused by f. sp. , is a damaging disease of wheat globally, and breeding resistant cultivars is the best control strategy. The Chinese winter wheat cultivar Shumai126 (SM126) exhibited strong resistance to f. sp. in the field for more than 10 years. The objective of this study was to identify and map quantitative trait loci (QTL) for resistance to stripe rust in a population of 154 recombinant inbred lines (RILs) derived from a cross between cultivars Taichang29 (TC29) and SM126. The RILs were tested in six field environments with a mixture of the Chinese prevalent races (CYR32, CYR33, CYR34, Zhong4, and HY46) of f. sp. and in growth chamber with race CYR34 and genotyped using the Wheat55K single nucleotide polymorphism (SNP) array. Six QTL were mapped on chromosomes 1BL, 2AS, 2AL, 6AS, 6BS, and 7BL, respectively. All QTL were contributed by SM126 except The and had major effects, explaining 27.00 to 39.91% and 11.89 to 17.11% of phenotypic variances, which may correspond to known resistance genes and , respectively. The , , and with minor effects are likely novel. was only detected based on growth chamber seedling data. Additive effects were detected for the combination of , , and . SNP markers linked to ( and ) and ( and -) were converted to breeder-friendly Kompetitive allele-specific PCR (KASP) markers that would facilitate the deployment of stripe rust resistance genes in wheat breeding.
条锈病由小麦条锈菌（Puccinia striiformis f. sp. tritici）引起，是一种在全球范围内对小麦造成严重危害的病害，培育抗病品种是最佳防治策略。中国冬小麦品种蜀麦126（SM126）在田间对小麦条锈菌表现出超过10年的强抗性。本研究的目的是在由品种太昌29（TC29）和SM126杂交产生的154个重组自交系（RIL）群体中鉴定并定位抗条锈病的数量性状位点（QTL）。这些RIL在六个田间环境中用中国流行的小麦条锈菌生理小种（CYR32、CYR33、CYR34、中4和HY46）的混合菌进行测试，并在生长室中用CYR34小种进行测试，同时使用小麦55K单核苷酸多态性（SNP）芯片进行基因分型。分别在1BL、2AS、2AL、6AS、6BS和7BL染色体上定位到6个QTL。除QYr.sau-6B外，所有QTL均由SM126贡献。QYr.sau-2AS和QYr.sau-6AS具有主效，分别解释表型变异的27.00%至39.91%和11.89%至17.11%，它们可能分别对应已知的抗性基因Yr26和Yr6。具有微效的QYr.sau-1BL、QYr.sau-2AL和QYr.sau-7BL可能是新的。QYr.sau-1BL仅基于生长室幼苗数据被检测到。检测到QYr.sau-2AS、QYr.sau-6AS和QYr.sau-7BL组合的加性效应。与QYr.sau-2AS（Xwmc673和Xgwm533）和QYr.sau-6AS（Xgwm181和Xgwm577）连锁的SNP标记被转化为对育种者友好的竞争性等位基因特异性PCR（KASP）标记，这将有助于在小麦育种中部署条锈病抗性基因。
