Li Yuqin, Hu Yanling, Jiang Yun, Zhou Qiang, He Yu, He Jingshu, Chen Xuejiao, Chen Xue, Jiang Bo, Hao Ming, Ning Shunzong, Yuan Zhongwei, Zhang Jinrui, Xia Chongjing, Wu Bihua, Feng Lihua, Zhang Lianquan, Liu Dengcai, Huang Lin
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Theor Appl Genet. 2023 Dec 13;137(1):5. doi: 10.1007/s00122-023-04505-9.
A novel major adult-plant stripe rust resistance QTL derived from cultivated emmer wheat was mapped to a 123.6-kb region on wheat chromosome 2BL. Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat. Identification of new sources of resistance and their utilization in breeding programs is the effectively control strategy. The objective of this study was to identify and genetically characterize the stripe rust resistance derived from the cultivated emmer accession AS286. A recombinant inbred line population, developed from a cross between the susceptible durum wheat line langdon and AS286, was genotyped using the Wheat55K single nucleotide polymorphism array and evaluated in field conditions with a mixture of the prevalent Chinese Pst races (CYR32, CYR33, CYR34, Zhong4, and HY46) and in growth chamber with race CYR34. Three QTLs conferring resistance were mapped on chromosomes 1BS, 2BL, and 5BL, respectively. The QYrAS286-1BS and QYrAS286-2BL were stable with major effects, explaining 12.91% to 18.82% and 11.31% to 31.43% of phenotypic variation, respectively. QYrAS286-5BL was only detected based on growth chamber seedling data. RILs harboring both QYrAS286-1BS and QYrAS286-2BL showed high levels of stripe rust resistance equal to the parent AS286. The QYrAS286-2BL was only detected at the adult-plant stage, which is different from previously named Yr genes and inherited as a single gene. It was further mapped to a 123.6-kb region using KASP markers derived from SNPs identified by bulked segregant RNA sequencing (BSR-Seq). The identified loci enrich our stripe rust resistance gene pool, and the flanking markers developed here could be useful in marker-assisted selection for incorporating QYrAS286-2BL into wheat cultivars.
一个源自栽培二粒小麦的新型成年植株条锈病抗性QTL被定位到小麦2BL染色体上一个123.6 kb的区域。由真菌病原菌条形柄锈菌小麦专化型（Pst）引起的条锈病是小麦最具毁灭性的病害之一。鉴定新的抗性来源并将其应用于育种计划是有效的防治策略。本研究的目的是鉴定并从遗传角度表征源自栽培二粒小麦种质AS286的条锈病抗性。以感病硬粒小麦品系兰登与AS286杂交构建的重组自交系群体，利用小麦55K单核苷酸多态性芯片进行基因分型，并在田间条件下用中国流行的Pst小种（CYR32、CYR33、CYR34、中4和HY46）混合菌系以及在生长室中用CYR34小种进行评价。分别在1BS、2BL和5BL染色体上定位到3个赋予抗性的QTL。QYrAS286 - 1BS和QYrAS286 - 2BL具有稳定的主效作用，分别解释表型变异的12.91%至18.82%和11.31%至31.43%。QYrAS286 - 5BL仅根据生长室幼苗数据检测到。同时含有QYrAS286 - 1BS和QYrAS286 - 2BL的重组自交系表现出与亲本AS286相当的高水平条锈病抗性。QYrAS286 - 2BL仅在成年植株期检测到，这与之前命名的Yr基因不同，且按单基因遗传。利用通过混合分离RNA测序（BSR - Seq）鉴定的SNP衍生的KASP标记，将其进一步定位到一个123.6 kb的区域。鉴定出的位点丰富了我们的条锈病抗性基因库，这里开发的侧翼标记可用于标记辅助选择，将QYrAS286 - 2BL导入小麦品种。
