Jiang Yunfeng, Duan Luyao, Guan Fangnian, Yao Fangjie, Long Li, Wang Yuqi, Zhao Xuyang, Li Hao, Li Wei, Xu Qiang, Jiang Qiantao, Wang Jirui, Wei Yuming, Ma Jian, Kang Houyang, Qi Pengfei, Deng Mei, Zheng Youliang, Chen Guoyue
State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan 611130, P.R. China.
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, P.R. China.
Plant Dis. 2022 Apr;106(4):1209-1215. doi: 10.1094/PDIS-08-21-1618-RE. Epub 2022 Mar 29.
Stripe rust caused by f. sp. is one of the most destructive diseases of wheat. Identifying novel resistance genes applicable for developing disease-resistant cultivars is important for the sustainable control of wheat stripe rust. Chinese wheat landrace 'Xiaohemai' ('XHM') is an elite germplasm line with all-stage resistance (ASR) effective against predominant Chinese f. sp. races. In this study, we performed a bulked segregant analysis coupled with exome capture sequencing (BSE-seq) to identify a candidate genomic region strongly associated with stripe rust resistance on chromosome 1AL in 173 lines derived from the cross 'XHM' × 'Avocet S'. The gene, designated as , was validated by a conventional quantitative trait locus analysis using newly developed Kompetitive allele-specific PCR (KASP) markers, explaining up to 48.50% of the phenotypic variance. By testing a secondary mapping population comprising 144 lines from the same cross at the seedling stage with prevalent f. sp. race CYR34, was identified as a single Mendelian factor in a 1.5-cM interval flanked by KASP markers and . This region corresponded to a 5.76-Mb genomic interval on 'Chinese Spring' chromosome 1AL. Furthermore, two cosegregating KASP markers showed high polymorphisms among 130 Chinese wheat cultivars and could be used for marker-assisted selection. Because no other genes for ASR that originated from common wheat have been detected on chromosome 1AL, is likely a novel gene that can be incorporated into modern breeding materials to develop wheat cultivars with enhanced stripe rust resistance.
由条形柄锈菌小麦专化型引起的条锈病是小麦最具毁灭性的病害之一。鉴定适用于培育抗病品种的新抗性基因对于小麦条锈病的可持续防治至关重要。中国小麦地方品种‘小偃麦’（‘XHM’）是一个优良种质系，对中国主要的条形柄锈菌小麦专化型小种具有全生育期抗性（ASR）。在本研究中，我们进行了混合分组分析法结合外显子捕获测序（BSE-seq），以在由‘XHM’ב阿伏塞特S’杂交产生的173个品系中鉴定出与1AL染色体上条锈病抗性紧密相关的候选基因组区域。该基因被命名为 ，通过使用新开发的竞争性等位基因特异性PCR（KASP）标记进行传统数量性状位点分析进行了验证，可解释高达48.50%的表型变异。通过在苗期用流行的条形柄锈菌小麦专化型小种CYR34对包含来自同一杂交组合的144个品系的次级作图群体进行检测， 被确定为一个单孟德尔因子，位于KASP标记 和 侧翼的1.5厘摩区间内。该区域对应于‘中国春’1AL染色体上一个5.76兆碱基的基因组区间。此外，两个共分离的KASP标记在130个中国小麦品种中表现出高度多态性，可用于标记辅助选择。由于在1AL染色体上尚未检测到其他源自普通小麦的全生育期抗性 基因， 可能是一个新基因，可纳入现代育种材料中，以培育具有增强条锈病抗性的小麦品种。
