Sudhakara Prasad K, Cao Xiyue, Gao Ning, Jin Qijie, Sanjay Sharma T, Henao-Pabon Gilberto, Li XiuJun
Department of Chemistry & Biochemistry, University of Texas at El Paso, Texas, USA.
College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, PR China.
Sens Actuators B Chem. 2020 Feb 15;305. doi: 10.1016/j.snb.2019.127516. Epub 2019 Dec 2.
Due to the lack of specific early detection methods for pancreatic cancer, it usually goes undetected until it is advanced. By employing paper-based electrodes (PPE), herein we for the first time developed a disposable low-cost paper-based immunosensor for rapid early quantitative detection of pancreatic cancer with a new biomarker, pseudopodium-enriched atypical kinase one, SGK269 (PEAK1). The immunosensor was constructed by fabricating PPEs immobilized with the versatile nanomaterial graphene oxide for the incorporation of antibodies to form an immunosensing platform, without the need of complicated surface modification. After it was confirmed that the PPEs exhibited excellent electrochemical properties, a sandwich-type electrochemical immunosensor was subsequently constructed by employing graphene oxide layers immobilized with anti-PEAK1, and the antibody conjugated with gold nanoparticles (AuNPs-tagged-Anti PEAK1). Further, spectral and surface characteristic studies confirmed the formation of the immunosensing platform. The immunosensor for PEAK1 exhibited a wide linear range between 10 pg mL and 10 pg mL with a low limit of detection (LOD) of 10 pg mL. The obtained results point towards rapid, sensitive, and specific early diagnosis of pancreatic cancer at the point of care and other low-resource settings.
由于缺乏胰腺癌的特异性早期检测方法，胰腺癌通常在病情进展之前都未被发现。在此，我们通过使用纸质电极（PPE），首次开发了一种一次性低成本纸质免疫传感器，用于利用一种新的生物标志物——富含伪足的非典型激酶1，即SGK269（PEAK1）对胰腺癌进行快速早期定量检测。该免疫传感器通过制备固定有多功能纳米材料氧化石墨烯的PPE来构建，用于掺入抗体以形成免疫传感平台，无需复杂的表面修饰。在确认PPE表现出优异的电化学性能后，随后通过使用固定有抗PEAK1的氧化石墨烯层以及与金纳米颗粒偶联的抗体（金纳米颗粒标记的抗PEAK1）构建了一种夹心型电化学免疫传感器。此外，光谱和表面特性研究证实了免疫传感平台的形成。用于检测PEAK1的免疫传感器在10 pg/mL至100 pg/mL之间呈现出较宽的线性范围，检测限低至10 pg/mL。所获得的结果表明可在护理点和其他资源匮乏的环境中对胰腺癌进行快速、灵敏且特异的早期诊断。
