Moll Luís, Badosa Esther, Planas Marta, Feliu Lidia, Montesinos Emilio, Bonaterra Anna
Laboratory of Plant Pathology, Institute of Food and Agricultural Technology-CIDSAV-XaRTA, University of Girona, Girona, Spain.
LIPPSO, Department of Chemistry, University of Girona, Girona, Spain.
Front Microbiol. 2021 Nov 8;12:753874. doi: 10.3389/fmicb.2021.753874. eCollection 2021.
is a plant pathogen that was recently introduced in Europe and is causing havoc to its agriculture. This Gram-negative bacterium invades the host xylem, multiplies, and forms biofilm occluding the vessels and killing its host. In spite of the great research effort, there is no method that effectively prevents or cures hosts from infections. The main control strategies up to now are eradication, vector control, and pathogen-free plant material. Antimicrobial peptides have arisen as promising candidates to combat this bacterium due to their broad spectrum of activity and low environmental impact. In this work, peptides previously reported in the literature and newly designed analogs were studied for its bactericidal and antibiofilm activity against . Also, their hemolytic activity and effect on tobacco leaves when infiltrated were determined. To assess the activity of peptides, the strain IVIA 5387.2 with moderate growth, able to produce biofilm and susceptible to antimicrobial peptides, was selected among six representative strains found in the Mediterranean area (DD1, CFBP 8173, Temecula, IVIA 5387.2, IVIA 5770, and IVIA 5901.2). Two interesting groups of peptides were identified with bactericidal and/or antibiofilm activity and low-moderate toxicity. The peptides and with dual (bactericidal-antibiofilm) activity against the pathogen and moderate toxicity stand out as the best candidates to control diseases. Nevertheless, peptides with only antibiofilm activity and low toxicity are also promising agents as they could prevent the occlusion of xylem vessels caused by the pathogen. The present work contributes to provide novel compounds with antimicrobial and antibiofilm activity that could lead to the development of new treatments against diseases caused by
是一种植物病原体，最近被引入欧洲，正在对其农业造成严重破坏。这种革兰氏阴性细菌侵入宿主木质部，繁殖并形成生物膜，阻塞导管并杀死宿主。尽管进行了大量研究，但尚无有效预防或治愈宿主感染的方法。目前主要的控制策略是根除、控制传播媒介和使用无病原体的植物材料。抗菌肽因其广泛的活性谱和低环境影响而成为对抗这种细菌的有希望的候选物。在这项工作中，研究了文献中先前报道的肽和新设计的类似物对其的杀菌和抗生物膜活性。此外，还测定了它们的溶血活性以及渗入烟草叶片时的影响。为了评估肽的活性，在地中海地区发现的六个代表性菌株（DD1、CFBP 8173、特梅库拉、IVIA 5387.2、IVIA 5770和IVIA 5901.2）中，选择了生长适度、能够产生生物膜且对抗菌肽敏感的IVIA 5387.2菌株。鉴定出两组有趣的具有杀菌和/或抗生物膜活性且毒性低至中等的肽。对病原体具有双重（杀菌-抗生物膜）活性且毒性中等的肽和脱颖而出，是控制疾病的最佳候选物。然而，仅具有抗生物膜活性且毒性低的肽也是有前途的药物，因为它们可以防止病原体引起的木质部导管阻塞。目前的工作有助于提供具有抗菌和抗生物膜活性的新型化合物，这可能会导致开发针对由引起的疾病的新疗法
