Peng Xin, Tun Win, Dai Shuang-Feng, Li Jia-Yue, Zhang Qun-Jie, Yin Guo-Ying, Yoon Jinmi, Cho Lae-Hyeon, An Gynheung, Gao Li-Zhi
Institution of Genomics and Bioinformatics, South China Agricultural University, Guangzhou, China.
Crop Biotech Institute, Graduate School of Biotechnology, Kyung Hee University, Yongin, South Korea.
Front Plant Sci. 2021 Nov 8;12:736419. doi: 10.3389/fpls.2021.736419. eCollection 2021.
Photoperiod sensitivity is a dominant determinant for the phase transition in cereal crops. () transcription factors (TFs) are involved in many physiological functions including the regulation of the photoperiodic flowering. However, the functional roles of TFs have not been elucidated in the wild progenitors of crops. In this study, we identified 41 TFs, including 19 , 17 , and five TFs in , the presumed wild ancestor of Asian cultivated rice. There are thirty-eight orthologous genes in , of which ten pairs of duplicated TFs are shared with . We investigated daily expression patterns, showing that 36 genes exhibited circadian rhythmic expression. A total of thirteen genes were identified as putative flowering suppressors in based on rhythmic and developmental expression patterns and transgenic phenotypes. We propose that , , and are the strong functional alleles of rice , , and , respectively. The SD treatment at 80 DAG stimulated flowering of the LD-grown plants. Our results further showed that the nine genes were significantly downregulated under the treatment. Our findings would provide valuable information for the construction of photoperiodic flowering regulatory network and functional characterization of the TFs in both and .
光周期敏感性是谷类作物阶段转变的主要决定因素。（）转录因子（TFs）参与许多生理功能，包括光周期开花的调控。然而，TFs在作物野生祖先中的功能作用尚未阐明。在本研究中，我们在亚洲栽培稻的假定野生祖先中鉴定出41个TFs，包括19个、17个和5个TFs。中有38个直系同源基因，其中10对重复的TFs与共有。我们研究了每日表达模式，表明36个基因表现出昼夜节律表达。基于节律性和发育表达模式以及转基因表型，总共13个基因被鉴定为中的假定开花抑制因子。我们提出，、和分别是水稻、和的强功能等位基因。在80日龄时进行短日照处理刺激了长日照生长的植株开花。我们的结果进一步表明，在该处理下9个基因显著下调。我们的发现将为光周期开花调控网络的构建以及和中TFs的功能表征提供有价值的信息。
