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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Parry D G, Taylor K W

Biochem J. 1978 Mar 15;170(3):523-7. doi: 10.1042/bj1700523.

Rabbit islets of Langerhans were disrupted by ultrasonic methods and the sonicated preparations were used to study proinsulin biosynthesis. 2. When [3h]leucine is incubated in such preparations, incorporation takes place into proinsulin, as evidenced by characterization on polyacrylamide gels, and by the conversion of this labelled material into insulin, by using trypsin. 3. The labelled proinsulin may also be purified by antiinsulin antibody bound to Sepharose. 4. With the broken-cell preparation it was shown that incorporation of leucine is accelerated by increasing the glucose content of the medium from 2mM to 16mM. However, 16mM-galactose or -sucrose did not stimulate incorporation significantly from basal values. This effect of glucose was abolished by cycloheximide. 5. The significance of these findings in relation to the mechanism of glucose stimulation of proinsulin biosynthesis is discussed.

采用超声方法破坏兔胰岛，将超声处理后的制剂用于研究胰岛素原的生物合成。2. 当在这类制剂中孵育[3H]亮氨酸时，亮氨酸会掺入胰岛素原，这通过聚丙烯酰胺凝胶电泳鉴定以及用胰蛋白酶将这种标记物质转化为胰岛素得以证明。3. 标记的胰岛素原也可用结合到琼脂糖上的抗胰岛素抗体进行纯化。4. 用破碎细胞制剂表明，将培养基中的葡萄糖含量从2mM增加到16mM可加速亮氨酸的掺入。然而，16mM的半乳糖或蔗糖与基础值相比并未显著刺激掺入。环己酰亚胺可消除葡萄糖的这种作用。5. 讨论了这些发现与葡萄糖刺激胰岛素原生物合成机制的相关性。

Parry D G, Taylor K W

Biochem J. 1978 Mar 15;170(3):523-7. doi: 10.1042/bj1700523.

Rabbit islets of Langerhans were disrupted by ultrasonic methods and the sonicated preparations were used to study proinsulin biosynthesis. 2. When [3h]leucine is incubated in such preparations, incorporation takes place into proinsulin, as evidenced by characterization on polyacrylamide gels, and by the conversion of this labelled material into insulin, by using trypsin. 3. The labelled proinsulin may also be purified by antiinsulin antibody bound to Sepharose. 4. With the broken-cell preparation it was shown that incorporation of leucine is accelerated by increasing the glucose content of the medium from 2mM to 16mM. However, 16mM-galactose or -sucrose did not stimulate incorporation significantly from basal values. This effect of glucose was abolished by cycloheximide. 5. The significance of these findings in relation to the mechanism of glucose stimulation of proinsulin biosynthesis is discussed.

采用超声方法破坏兔胰岛，将超声处理后的制剂用于研究胰岛素原的生物合成。2. 当在这类制剂中孵育[3H]亮氨酸时，亮氨酸会掺入胰岛素原，这通过聚丙烯酰胺凝胶电泳鉴定以及用胰蛋白酶将这种标记物质转化为胰岛素得以证明。3. 标记的胰岛素原也可用结合到琼脂糖上的抗胰岛素抗体进行纯化。4. 用破碎细胞制剂表明，将培养基中的葡萄糖含量从2mM增加到16mM可加速亮氨酸的掺入。然而，16mM的半乳糖或蔗糖与基础值相比并未显著刺激掺入。环己酰亚胺可消除葡萄糖的这种作用。5. 讨论了这些发现与葡萄糖刺激胰岛素原生物合成机制的相关性。