Jain K, Logothetopoulos J
Endocrinology. 1977 Apr;100(4):923-7. doi: 10.1210/endo-100-4-923.
Inosine and guanosine were potent stimuli of proinsulin biosynthesis ([3H]leucine incorporation) in isolated pancreatic islets of the rat. The effect was nearly abolished by formycin B, an inhibitor of purine nucleoside phosphorylase, but not by D-mannoheptulose. The corresponding bases had no effect on the rate of proinsulin biosynthesis. D-ribose enhance proinsulin biosynthesis at low concentrations )0.3-0.6mM) but concentrations above 5 mM were ineffective. The effect of all three compounds was highly specific for proinsulin biosynthesis, since incorporation of [3H]leucine into other islet proteins was not significantly stimulated. The data strongly indicate that metabolic signals regulate modulation of proinsulin biosynthesis in the beta cells.
肌苷和鸟苷是大鼠分离胰岛中胰岛素原生物合成（[3H]亮氨酸掺入）的有效刺激物。嘌呤核苷磷酸化酶抑制剂博来霉素B几乎消除了这种作用，但D-甘露庚酮糖没有此作用。相应的碱基对胰岛素原生物合成速率没有影响。D-核糖在低浓度（0.3-0.6mM）时增强胰岛素原生物合成，但浓度高于5mM时无效。这三种化合物对胰岛素原生物合成的作用具有高度特异性，因为[3H]亮氨酸掺入其他胰岛蛋白并未受到显著刺激。数据有力地表明，代谢信号调节β细胞中胰岛素原生物合成的调节。
