Aydemir Ozkan, Mensah Benedicta, Marsh Patrick W, Abuaku Benjamin, Myers-Hansen James Leslie, Bailey Jeffrey A, Ghansah Anita
Department of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Brown University, Providence, RI, United States of America.
Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
PeerJ. 2021 Nov 9;9:e11794. doi: 10.7717/peerj.11794. eCollection 2021.
Antimalarial resistance surveillance in sub-Saharan Africa is often constrained by logistical and financial challenges limiting its breadth and frequency. At two sites in Ghana, we have piloted a streamlined sample pooling process created immediately by sequential addition of positive malaria cases at the time of diagnostic testing. This streamlined process involving a single tube minimized clinical and laboratory work and provided accurate frequencies of all known drug resistance mutations after high-throughput targeted sequencing using molecular inversion probes. Our study validates this method as a cost-efficient, accurate and highly-scalable approach for drug resistance mutation monitoring that can potentially be applied to other infectious diseases such as tuberculosis.
撒哈拉以南非洲地区的抗疟药物耐药性监测常常受到后勤和资金方面的挑战限制，影响了监测的广度和频率。在加纳的两个地点，我们试点了一种简化的样本合并流程，即在诊断检测时通过依次添加阳性疟疾病例即时创建样本池。这个简化流程只需一根管子，将临床和实验室工作减到最少，并在使用分子倒置探针进行高通量靶向测序后，提供了所有已知耐药性突变的准确频率。我们的研究验证了这种方法是一种经济高效、准确且高度可扩展的耐药性突变监测方法，有可能应用于结核病等其他传染病。
