酿酒酵母中线粒体蛋白质合成产物的稳定性。一种测定蛋白质半衰期的新方法。
The lability of the products of mitochondrial protein synthesis in Saccharomyces cerevisiae. A novel method for protein half-life determination.
作者信息
Bakalkin G Y, Kalnov S L, Galkin A V, Zubatov A S, Luzikov V N
出版信息
Biochem J. 1978 Mar 15;170(3):569-76. doi: 10.1042/bj1700569.
Abstract
A method for the determination of the half-life of mitochondrial translation products in yeast in vivo is proposed. The method uses inhibitors of cytoplasmic and mitochondrial protein synthesis and is based on double-labelling pulse-chase techniques, the second label being used to estimate 'post-incorporation' during the 'chase'. For the first time the difference between post-incroporation and the widely known recycling of the label is considered. These studies show that, in the turnover of mitochondrial translation products, the problem is of post-incorporation into mitochondria (especially from the cell sap) is predominant. The results obtained with this procedure indicate that the half-life of the products of mitochondrial protein synthesis in yeast at the late-exponential phase is about 60 min. The results suggest that mitochondrial transplantation products are subject to proteolysis to acid-soluble forms.
摘要
本文提出了一种测定酵母体内线粒体翻译产物半衰期的方法。该方法使用细胞质和线粒体蛋白质合成抑制剂，基于双标记脉冲追踪技术，第二个标记用于估计“追踪”过程中的“掺入后”情况。首次考虑了掺入后与广为人知的标记循环之间的差异。这些研究表明，在线粒体翻译产物的周转中，掺入线粒体（尤其是从细胞液中）的问题占主导地位。用该程序获得的结果表明，酵母在指数后期线粒体蛋白质合成产物的半衰期约为60分钟。结果表明线粒体移植产物会被蛋白水解成酸溶性形式。
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The lability of the products of mitochondrial protein synthesis in Saccharomyces cerevisiae. A novel method for protein half-life determination.酿酒酵母中线粒体蛋白质合成产物的稳定性。一种测定蛋白质半衰期的新方法。
Biochem J. 1978 Mar 15;170(3):569-76. doi: 10.1042/bj1700569.
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引用本文的文献
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Correlation between the rate of proteolysis of mitochondrial translation products and fluidity of the mitochondrial inner membrane in Saccharomyces cerevisiae yeast. Alteration of the rate of proteolysis under glucose repression.酿酒酵母中线粒体翻译产物的蛋白水解速率与线粒体内膜流动性之间的相关性。葡萄糖阻遏下蛋白水解速率的改变。
Biochem J. 1983 Sep 15;214(3):785-94. doi: 10.1042/bj2140785.
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Biochem J. 1991 Feb 15;274 ( Pt 1)(Pt 1):199-205. doi: 10.1042/bj2740199.
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Proteolysis of the products of mitochondrial protein synthesis in yeast mitochondria and submitochondrial particles.酵母线粒体和亚线粒体颗粒中蛋白质合成产物的蛋白水解作用。
Biochem J. 1979 Jul 15;182(1):195-202. doi: 10.1042/bj1820195.
本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
[Study of yeast mitochondria. I. Variations in mitochondrial ultrastructure during the aerobic growth cycle].[酵母线粒体的研究。I. 需氧生长周期中线粒体超微结构的变化]
J Ultrastruct Res. 1962 Aug;7:121-40. doi: 10.1016/s0022-5320(62)80031-8.
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Biochim Biophys Acta. 1961 Aug 5;51:356-60. doi: 10.1016/0006-3002(61)90177-9.
4
Electrophoretic pattern of and amino acid incorporation in vitro into the insoluble mitochondrial protein of neurospora crassa wild type and mi-1 mutant.粗糙脉孢菌野生型和mi-1突变体不溶性线粒体蛋白的体外电泳图谱及氨基酸掺入情况
FEBS Lett. 1968 Sep;1(4):235-240. doi: 10.1016/0014-5793(68)80071-7.
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Turnover of outer and inner membrane proteins of rat liver mitochondria.大鼠肝脏线粒体内膜和外膜蛋白的周转
FEBS Lett. 1968 Aug;1(3):153-155. doi: 10.1016/0014-5793(68)80045-6.
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J Biol Chem. 1967 Oct 25;242(20):4584-6.
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Turnover of mitochondrial components of normal and essential fatty acid-deficient rats.正常和必需脂肪酸缺乏大鼠线粒体成分的更新
Biochem J. 1967 Sep;104(3):1026-32. doi: 10.1042/bj1041026.
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Delta-aminolevulinic acid synthetase. II. Induction in rat liver.δ-氨基乙酰丙酸合成酶。II. 大鼠肝脏中的诱导作用。
J Biol Chem. 1966 Oct 10;241(19):4323-9.
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J Biol Chem. 1969 Jun 25;244(12):3303-15.
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