Mir Mohammad Imran, Kumar B Kiran, Gopalakrishnan Subramaniam, Vadlamudi Srinivas, Hameeda Bee
Department of Botany, UCS, Osmania University, Hyderabad, 500007, Telangana, India.
International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Hyderabad, 502319, Telangana, India.
Heliyon. 2021 Nov 5;7(11):e08321. doi: 10.1016/j.heliyon.2021.e08321. eCollection 2021 Nov.
Six rhizobia-like-bacterial strains in total, secluded from the root and stem nodules of various leguminous plants were characterized for growth promoting ability on ICCV 2 variety of chickpea. Bacterial strains showed production of IAA, NH, siderophore, HCN, ACC deaminase, hydrolytic enzyme production such as chitinase, amylase, protease, lipase, β-1, 3-glucanase and solubilization of nutrients such as phosphate, zinc and potassium. However the performance of PGP traits characterized varied among the six bacterial strains. The sequences of 16S rRNA gene of bacterial strains IHSR, IHRG, IHAA, IHGN-3, IHCP-1 and IHCP-2 showed maximum identity with sp., , , sp., and . In plate culture conditions the bacterial strains changed the colour of media (NFB) from green to blue and showed amplification of gene by PCR, and also enhanced nodule formation in chickpea under greenhouse conditions, which explains their nitrogen fixing ability. Scanning electron microscopy studies of chickpea roots showed colonization by all the six bacterial strains in solo and by consortium (IHRG + IHGN-3). Under greenhouse conditions, chickpea plants inoculated with different strains showed improvement in plant height, number of branches, total chlorophyll, nodule number, nodule weight, shoot weight, root weight, root volume and root surface area at 30 and 45 days after sowing (DAS) over the uninoculated control plants. It was also observed at the crop maturity stage all the bacterial strains inoculated separately enhanced pod number, seed number and total NPK compared to uninoculated control plants. This study suggests that bacteria associated with root and stem nodules can be a promising resource to enhance nodulation, PGP and crop yields in chickpea.
总共从各种豆科植物的根瘤和茎瘤中分离出6株类根瘤菌菌株，对其在鹰嘴豆ICCV 2品种上的促生长能力进行了表征。这些细菌菌株表现出产生吲哚-3-乙酸（IAA）、氨、铁载体、氰化氢（HCN）、1-氨基环丙烷-1-羧酸（ACC）脱氨酶，以及产生水解酶，如几丁质酶、淀粉酶、蛋白酶、脂肪酶、β-1,3-葡聚糖酶，还能使磷酸盐、锌和钾等养分溶解。然而，所表征的植物生长促进（PGP）特性在这6株细菌菌株中的表现各不相同。细菌菌株IHSR、IHRG、IHAA、IHGN-3、IHCP-1和IHCP-2的16S rRNA基因序列与 种、 种、 种、 种、 种和 种具有最高同源性。在平板培养条件下，这些细菌菌株使培养基（NFB）的颜色从绿色变为蓝色，并通过聚合酶链反应（PCR）显示 基因扩增，而且在温室条件下还增强了鹰嘴豆的结瘤，这解释了它们的固氮能力。鹰嘴豆根的扫描电子显微镜研究表明，所有6株细菌菌株单独以及混合（IHRG + IHGN-3）时均能在根上定殖。在温室条件下，接种不同菌株的鹰嘴豆植株在播种后30天和45天，与未接种的对照植株相比，株高、分枝数、总叶绿素、根瘤数、根瘤重量、地上部重量、根重量、根体积和根表面积均有所改善。在作物成熟阶段还观察到，与未接种的对照植株相比，所有单独接种的细菌菌株均增加了荚果数、种子数和总氮磷钾含量。这项研究表明，与根瘤和茎瘤相关的细菌可能是增强鹰嘴豆结瘤、植物生长促进和作物产量的有前景的资源。
