de Marcos Carpio Isabel, Sanghani-Kerai Anita, Solano Miguel A, Blunn Gordon, Jifcovici Alexandra, Fitzpatrick Noel
Fitzpatrick Referrals, Surrey GU7 2QQ, UK.
School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth PO1 2DT, UK.
Vet Sci. 2021 Nov 3;8(11):262. doi: 10.3390/vetsci8110262.
Due to its easy preparation and that it is well tolerated, the use of autologous platelet-rich plasma (PRP) has become increasingly popular in regenerative medicine. However, there are still no clear guidelines on how it should be classified or whether the individual canine patient's clinical status can influence its quality.
This study aims to show if the weight, age, sex, neutered status or breed of canine patients have any correlation with the composition of PRP.
A blinded count of the platelets and white blood cells (WBC) was performed from 111 samples from 92 client owned dogs undergoing treatment for degenerative joint disease (DJD). The results were analysed using Pearson correlation test, ANOVA test or Student -test.
There is a positive correlation between the number of platelets and WBC in canine patients of different breeds, but there was no significant difference on the platelet number and WBC number among the different breeds. The weight of the patient is also directly correlated to the platelet number ( = 0.003) but not WBC number. WBC number was negatively correlated to the weight of the patient. The sex and age of the patient did not affect platelets and WBC number, although WBC number is increased in non-neutered male population ( = 0.003). However, it would be interesting to investigate whether the growth factors released from the platelet granules are affected by patient variables in a canine population.
Our results show that it is possible to obtain good quality autologous PRP, irrespective of age, sex, neutered status or weight of the patient, for PRP regenerative therapy.
由于易于制备且耐受性良好，自体富血小板血浆（PRP）在再生医学中的应用越来越广泛。然而，关于其应如何分类，以及个体犬类患者的临床状况是否会影响其质量，目前仍没有明确的指导方针。
本研究旨在表明犬类患者的体重、年龄、性别、绝育状态或品种与PRP的成分是否存在任何相关性。
对92只因退行性关节病（DJD）接受治疗的客户拥有的犬只的111份样本进行血小板和白细胞（WBC）的盲法计数。使用Pearson相关检验、方差分析或学生检验对结果进行分析。
不同品种犬类患者的血小板数量和白细胞数量之间存在正相关，但不同品种之间的血小板数量和白细胞数量没有显著差异。患者的体重也与血小板数量直接相关（P = 0.003），但与白细胞数量无关。白细胞数量与患者体重呈负相关。患者的性别和年龄不影响血小板和白细胞数量，尽管未绝育雄性犬的白细胞数量增加（P = 0.003）。然而，研究血小板颗粒释放的生长因子是否受犬类群体中患者变量的影响将是有趣的。
我们的结果表明，对于PRP再生治疗，无论患者的年龄、性别、绝育状态或体重如何，都有可能获得高质量的自体PRP。
