Yoon Jungho, Park Taemook, Kim Ahram, Song Heeeun, Park Byung-Joo, Ahn Hee-Seop, Go Hyeon-Jeong, Kim Dong-Hwi, Lee Joong-Bok, Park Seung-Yong, Song Chang-Seon, Lee Sang-Won, Choi In-Soo
Equine Clinic, Jeju Stud Farm, Korea Racing Authority, Jeju 63346, Korea.
Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
Vet Sci. 2021 Nov 7;8(11):268. doi: 10.3390/vetsci8110268.
Equine parvovirus-cerebrospinal fluid (EqPV-CSF) and eqcopivirus (EqCoPV) are new parvovirus species (EqPVs) identified from various tissues (CSF, blood, and respiratory swabs) in horses with neurologic and respiratory diseases. In this study, we described the prevalence rate of EqPV-CSF and EqCoPV in 133 and 77 serum and fecal samples, respectively, using polymerase chain reaction. Further, we analyzed the potential risk factors for infection. We calculated the nucleotide and amino acid similarity and constructed phylogenetic trees. There was a moderate-to-high prevalence rate (EqPV-CSF: 3.8%; EqCoPV 9.8%) of each virus in serum; moreover, age, country of foaling, and clinical colic signs were significantly associated with the EqPVs infection. The newly identified EqPV-CSF/EqCoPV genomes had high nucleotide and amino acid identities with previously isolated strains in the USA. In phylogenetic analysis, they clustered and formed a new subgroup in the genus . To our knowledge, this is the first field epidemiologic study on EqPV-CSF and EqCoPV using both serum and fecal samples. Our findings demonstrate the risk factors for infection and could facilitate the development of disease prevention strategies.
马细小病毒 - 脑脊液（EqPV - CSF）和马科细小病毒（EqCoPV）是从患有神经和呼吸系统疾病的马匹的各种组织（脑脊液、血液和呼吸道拭子）中鉴定出的新型细小病毒种类（EqPVs）。在本研究中，我们分别使用聚合酶链反应描述了133份血清样本和77份粪便样本中EqPV - CSF和EqCoPV的流行率。此外，我们分析了感染的潜在风险因素。我们计算了核苷酸和氨基酸相似性并构建了系统发育树。血清中每种病毒的流行率为中度至高度（EqPV - CSF：3.8%；EqCoPV：9.8%）；此外，年龄、出生国家和临床绞痛体征与EqPVs感染显著相关。新鉴定的EqPV - CSF/EqCoPV基因组与美国先前分离的毒株具有高核苷酸和氨基酸同一性。在系统发育分析中，它们聚类并在该属中形成一个新的亚组。据我们所知，这是首次使用血清和粪便样本对EqPV - CSF和EqCoPV进行的现场流行病学研究。我们的研究结果证明了感染的风险因素，并有助于制定疾病预防策略。
