China Institute of Veterinary Drug Control, Beijing 100081, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong 271001, China.
Poult Sci. 2022 Jan;101(1):101540. doi: 10.1016/j.psj.2021.101540. Epub 2021 Oct 20.
Group-I Fowl adenovirus (FAdV) is still widespread in China's chicken farms, leading to huge economic losses. The traditional PCR method, which can detect all serotypes at the same time, is not sensitive enough to obtain accurate results, especially in some samples containing only a low titer of virus, such as contaminated live vaccine. In order to solve this problem, this study developed a dot blot assay based on the above PCR method. A total of 6 probes targeting the conserved region of FAdV were designed and systematically optimized through sensitivity, accuracy, and stability analyses. Results showed that it is not only suitable for 12 serotypes, but also effectively improve the sensitivity, which increased more than 100 times in comparison with PCR assay. Moreover, this sensitivity was increased 100 times when detecting contaminated live vaccine samples, showing the great prospect of this method in daily monitoring.
群 I 禽腺病毒（FAdV）在中国的鸡场仍广泛存在，导致巨大的经济损失。传统的 PCR 方法可以同时检测所有血清型，但不够敏感，无法获得准确的结果，尤其是在一些病毒滴度较低的样本中，如污染的活疫苗。为了解决这个问题，本研究在上述 PCR 方法的基础上开发了斑点印迹分析。总共设计了 6 种针对 FAdV 保守区域的探针，并通过灵敏度、准确性和稳定性分析进行了系统优化。结果表明，该方法不仅适用于 12 种血清型，而且有效提高了灵敏度，与 PCR 检测相比提高了 100 多倍。此外，当检测污染的活疫苗样本时，灵敏度提高了 100 倍，显示出该方法在日常监测中的广阔前景。
