College of Chemistry & Environmental Science, Guangdong Ocean University, Zhanjiang, 524088, China.
College of Chemistry & Environmental Science, Guangdong Ocean University, Zhanjiang, 524088, China.
Carbohydr Polym. 2022 Jan 15;276:118715. doi: 10.1016/j.carbpol.2021.118715. Epub 2021 Oct 2.
Four fractions of water-extracted Sepia esculenta ink polysaccharides (SIP) were separated by dicthylaminoethy (DEAE) cellulose chromatography. The eluted fraction with the highest yield was characterized as a sulfate-rich glycosaminoglycan named SIP-IV. According to the analysis of laser scattering and refractive index signals, SIP-IV was determined to be 14.4 kDa and spherical molecular conformation in salt solution. SIP-IV is composed of fucose, galactosamine, glucosamine, mannose and glucuronic acid with a molar ratio of 5.1:7.3:3.8:1:4.4, which is obviously different from reported SIPs. SIP-IV promoted yeast proliferation and intercellular antioxidant level. Based on multi-omics strategy, data of transcriptome analysis suggested that growth promotion of SIP-IV on Saccharomyces cerevisiae might be attributed to regulation of Rho protein signal transduction, nuclear autophagy and nitrogen utilization. Combined with the metabolome results, SIP-IV also re-profiled metabolism of amino acids and phospholipids in yeast cells.
四种水提乌贼墨多糖（SIP）级分经二乙氨基乙基（DEAE）纤维素层析分离，洗脱得率最高的级分鉴定为富含硫酸基团的糖胺聚糖，命名为 SIP-IV。根据激光散射和折射率信号分析，SIP-IV 在盐溶液中呈 14.4 kDa 的球形分子构象。SIP-IV 由岩藻糖、半乳糖胺、氨基葡萄糖、甘露糖和葡萄糖醛酸组成，摩尔比为 5.1:7.3:3.8:1:4.4，与报道的 SIP 明显不同。SIP-IV 促进酵母增殖和细胞间抗氧化水平。基于多组学策略，转录组数据分析表明，SIP-IV 对酿酒酵母的促生长作用可能归因于 Rho 蛋白信号转导、核自噬和氮利用的调节。结合代谢组学结果，SIP-IV 还重新分析了酵母细胞中氨基酸和磷脂的代谢。
