Key Laboratory of Chemical Biology (Ministry of Education), Institute of Biochemical and Biotechnological Drugs, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China.
Department of Pharmacy, Qilu Hospital, Shandong University, Jinan 250012, Shandong, China.
Int J Biol Macromol. 2023 May 15;237:124040. doi: 10.1016/j.ijbiomac.2023.124040. Epub 2023 Mar 16.
Sepiella maindroni ink polysaccharide (SIP) from the ink of cuttlefish Sepiella maindroni and its sulfated derivative (SIP-SII) have been demonstrated to possess diverse biological activities. But little is known about low molecular weight squid ink polysaccharides (LMWSIPs). In this study, LMWSIPs were prepared by acidolysis, and the fragments with molecular weight (M) distribution in the ranges of 7 kDa to 9 kDa, 5 kDa to 7 kDa and 3 kDa to 5 kDa were grouped and named as LMWSIP-1, LMWSIP-2 and LMWSIP-3, respectively. The structural features of LMWSIPs were elucidated, and their anti-tumor, antioxidant and immunomodulatory activities were also studied. The results showed that with the exception of LMWSIP-3, the main structures of LMWSIP-1 and LMWSIP-2 did not change compared with SIP. Though there were no significant differences in the antioxidant capacity between LMWSIPs and SIP, the anti-tumor and immunomodulatory activities of SIP were enhanced to a certain extent after degradation. It is particularly noteworthy that the activities of LMWSIP-2 in anti-proliferation, promoting apoptosis and inhibiting migration of tumor cells as well as promoting the proliferation of spleen lymphocytes were significantly higher than those of SIP and the other degradation products, which is promising in the anti-tumor pharmaceutical field.
鱿鱼墨低分子量多糖（LMWSIPs）的研究较少。本研究采用酸解法制备 LMWSIPs，将分子量分布在 7 kDa 到 9 kDa、5 kDa 到 7 kDa 和 3 kDa 到 5 kDa 范围内的片段分别进行分组并命名为 LMWSIP-1、LMWSIP-2 和 LMWSIP-3。对 LMWSIPs 的结构特征进行了阐明，并研究了其抗肿瘤、抗氧化和免疫调节活性。结果表明，除了 LMWSIP-3 之外，LMWSIP-1 和 LMWSIP-2 的主要结构与 SIP 相比没有变化。尽管 LMWSIPs 和 SIP 的抗氧化能力没有显著差异，但 SIP 经降解后抗肿瘤和免疫调节活性得到了一定程度的增强。值得特别关注的是，LMWSIP-2 在抑制肿瘤细胞增殖、促进凋亡和迁移以及促进脾淋巴细胞增殖方面的活性均显著高于 SIP 和其他降解产物，这在抗肿瘤药物领域具有广阔的应用前景。
