College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, China, Harbin 150040, Heilongjiang, China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, Heilongjiang, China; Key Laboratory of Bio-Based Material Science and Technology of Ministry of Education Northeast Forestry University, Harbin 150040, Heilongjiang, China.
College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, China, Harbin 150040, Heilongjiang, China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, Heilongjiang, China.
Carbohydr Polym. 2022 Jan 15;276:118788. doi: 10.1016/j.carbpol.2021.118788. Epub 2021 Oct 21.
The purpose of this study was to investigate the potential of bacterial cellulose nanofiber suspension (BCNs) as stabilizer in anti-solvent precipitation and its effect on improving bioavailability of coenzyme Q10. Bacterial cellulose (BC) was hydrolyzed by sulfuric acid followed by the oxidation with hydrogen peroxide to prepare BCNs. The suspension of BCNs-loaded CoQ10 (CoQ10-BCNs) were prepared by antisolvent precipitation. The zeta potential of CoQ10-BCNs was about -36.01 mV. The properties of CoQ10, BCNs and CoQ10-BCNs were studied by scanning electron microscopy, transmission electron microscope, Fourier-transform infrared spectroscopy, X-ray diffraction, differential scanning calorimetry and thermo gravimetric analysis. The crystallinity of CoQ10 decreased in CoQ10-BCNs compared with the raw CoQ10, and CoQ10-BCNs have good physicochemical stability. In oral bioavailability studies, the area under curve (AUC) of CoQ10-BCNs was about 3.62 times higher than the raw CoQ10 in rats.
本研究旨在探讨细菌纤维素纳米纤维悬浮液（BCNs）作为抗溶剂沉淀稳定剂的潜力及其对提高辅酶 Q10 生物利用度的影响。通过硫酸水解和过氧化氢氧化制备 BCNs。通过抗溶剂沉淀法制备负载 CoQ10 的 BCNs 悬浮液（CoQ10-BCNs）。CoQ10-BCNs 的zeta 电位约为-36.01 mV。通过扫描电子显微镜、透射电子显微镜、傅里叶变换红外光谱、X 射线衍射、差示扫描量热法和热重分析研究了 CoQ10、BCNs 和 CoQ10-BCNs 的性质。与原 CoQ10 相比，CoQ10-BCNs 中的 CoQ10 结晶度降低，且 CoQ10-BCNs 具有良好的物理化学稳定性。在口服生物利用度研究中，CoQ10-BCNs 的曲线下面积（AUC）约为大鼠中原 CoQ10 的 3.62 倍。
