Zhou Huafeng, Zhang Jing, Long Yingfang, Liu Guoqing, Duan Mingxing, Xia Qiang
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
J Nanosci Nanotechnol. 2013 Jan;13(1):706-10. doi: 10.1166/jnn.2013.7089.
Coenzyme Q10-loaded lecithin nanocapsules (CoQ10-LNCs), composed of a CoQ10/lecithin/ GTCC/glycerol aqueous solution, were prepared by high-pressure homogenization. The zeta potential of the CoQ10-LNCs above -60 mV was determined on a Malvern Zetasize 2000 (Malvern Instruments, UK). The spherical shape of the CoQ10-LNCs was observed by using freeze-fracture transmission electron microscopy (FF-TEM), and the particle size was found to be below 100 nm. The supercooled state of the CoQ10-LNCs was observed by differential scanning calorimetry (DSC). In an oral bioavailability study, the CoQ10 plasma level after administering CoQ10-LNCs was higher than that after administering a CoQ10 tablet over 24 hours, and the relative bioavailability of CoQ10 was improved to 176.6% in mice. Based on the above results, the LNC delivery system might be a potential vehicle for improving the oral bioavailability of CoQ10.
由辅酶Q10/卵磷脂/甘油三乙酸酯/甘油水溶液组成的辅酶Q10负载卵磷脂纳米胶囊（CoQ10-LNCs）通过高压均质法制备。在英国马尔文仪器公司的马尔文Zetasize 2000上测定CoQ10-LNCs的ζ电位高于-60 mV。通过冷冻断裂透射电子显微镜（FF-TEM）观察CoQ10-LNCs的球形形状，发现粒径低于100 nm。通过差示扫描量热法（DSC）观察CoQ10-LNCs的过冷状态。在一项口服生物利用度研究中，给予CoQ10-LNCs后24小时内辅酶Q10的血浆水平高于给予辅酶Q10片剂后的水平，并且辅酶Q10在小鼠中的相对生物利用度提高到176.6%。基于上述结果，LNC递送系统可能是提高辅酶Q10口服生物利用度的潜在载体。
