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诱导狼疮病后 NZB/W F1 小鼠粪便微生物群的变化。

Fecal microbiota changes in NZB/W F1 mice after induction of lupus disease.

机构信息

Division of Rheumatology, Allergy and Immunology, Chang Gung Memorial Hospital, Taoyuan, Taiwan.

Division of Rheumatology, Allergy and Immunology, Chang Gung Memorial Hospital, Keelung, Taiwan.

出版信息

Sci Rep. 2021 Nov 25;11(1):22953. doi: 10.1038/s41598-021-02422-9.

DOI:10.1038/s41598-021-02422-9
PMID:34824318
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8616951/
Abstract

The association between the gut microbiota and the development of lupus is unclear. We investigated alterations in the gut microbiota after induction of lupus in a murine model using viral peptide of human cytomegalovirus (HCMV). Three treatment arms for the animals were prepared: intraperitoneal injection of HCMVpp65 peptide, adjuvant alone, and PBS injection. Feces were collected before and after lupus induction biweekly for 16S rRNA sequencing. HCMVpp65 peptide immunization induced lupus-like effects, with higher levels of anti-dsDNA antibodies, creatinine, proteinuria, and glomerular damage, compared with mice treated with nothing or adjuvant only. The Simpson diversity value was higher in mice injected with HCMVpp65 peptide, but there was no difference in ACE or Chao1 among the three groups. Statistical analysis of metagenomic profiles showed a higher abundance of various families (Saccharimonadaceae, Marinifiaceae, and Desulfovibrionaceae) and genera (Candidatus Saccharimonas, Roseburia, Odoribacter, and Desulfovibrio) in HCMVpp65 peptide-treated mice. Significant correlations between increased abundances of related genera (Candidatus Saccharimonas, Roseburia, Odoribacter, and Desulfovibrio) and HCMVpp65 peptide immunization-induced lupus-like effects were observed. This study provides insight into the changes in the gut microbiota after lupus onset in a murine model.

摘要

肠道微生物群与狼疮的发展之间的关系尚不清楚。我们使用人类巨细胞病毒（HCMV）的病毒肽在狼疮小鼠模型中研究了狼疮诱导后肠道微生物群的变化。为动物准备了三个治疗组：HCMVpp65 肽腹腔注射、佐剂单独注射和 PBS 注射。在狼疮诱导前和后每两周收集粪便进行 16S rRNA 测序。与仅用佐剂或 PBS 处理的小鼠相比，HCMVpp65 肽免疫接种诱导狼疮样效应，具有更高水平的抗 dsDNA 抗体、肌酐、蛋白尿和肾小球损伤。与仅用佐剂或 PBS 处理的小鼠相比，HCMVpp65 肽注射的小鼠 Simpson 多样性值更高，但三组间 ACE 或 Chao1 无差异。对宏基因组谱的统计分析显示，HCMVpp65 肽处理的小鼠中各种科（Saccharimonadaceae、Marinifiaceae 和 Desulfovibrionaceae）和属（Candidatus Saccharimonas、Roseburia、Odoribacter 和 Desulfovibrio）的丰度更高。相关属（Candidatus Saccharimonas、Roseburia、Odoribacter 和 Desulfovibrio）丰度增加与 HCMVpp65 肽免疫诱导狼疮样效应之间存在显著相关性。本研究为狼疮小鼠模型中肠道微生物群在狼疮发病后的变化提供了新的认识。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3542/8616951/a353ab2ec49e/41598_2021_2422_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3542/8616951/907bcaa4759c/41598_2021_2422_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3542/8616951/957c97e66789/41598_2021_2422_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3542/8616951/4a6383f08a0b/41598_2021_2422_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3542/8616951/a0190c7ffb5d/41598_2021_2422_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3542/8616951/2fbde9d73fbd/41598_2021_2422_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3542/8616951/a353ab2ec49e/41598_2021_2422_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3542/8616951/907bcaa4759c/41598_2021_2422_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3542/8616951/957c97e66789/41598_2021_2422_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3542/8616951/4a6383f08a0b/41598_2021_2422_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3542/8616951/a0190c7ffb5d/41598_2021_2422_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3542/8616951/2fbde9d73fbd/41598_2021_2422_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3542/8616951/a353ab2ec49e/41598_2021_2422_Fig6_HTML.jpg

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2
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Sci Transl Med. 2020 Jul 8;12(551). doi: 10.1126/scitranslmed.aax2220.
3
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Microorganisms. 2025 Mar 1;13(3):556. doi: 10.3390/microorganisms13030556.
4
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J Transl Autoimmun. 2025 Jan 7;10:100269. doi: 10.1016/j.jtauto.2025.100269. eCollection 2025 Jun.
5
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Nutrients. 2025 Jan 10;17(2):247. doi: 10.3390/nu17020247.
6
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7
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