Conder G A, Mayberry L F, Bristol J R, Castro G A, Lee B L, Kratzer D D, Folz S D, Rector D L
The Upjohn Company, Kalamazoo, MI 49001.
Prostaglandins. 1987 Dec;34(6):817-27. doi: 10.1016/0090-6980(87)90063-3.
PGE1 and PGE2 have been reported to enhance natural expulsion of Nippostrongylus brasiliensis, a nematode parasite, from the intestine of the rat. Mucus production may also be a key element of worm rejection. Our study attempts to determine if 1) PGE1 or PGE2 alter the normal course of infection with N. brasiliensis in rats, 2) a known mucous enhancing drug, acetazolamide, can augment the rate of worm expulsion, and 3) combinations of prostaglandins and acetazolamide affect N. brasiliensis in the rat. Rats were inoculated with approximately 1,000 infective larvae of N. brasiliensis. Animals were administered, intraduodenally, one of the following: 0.2 ml 0.9% NaCl; 0.2 ml 100% ethanol; 250 micrograms PGE1/0.2 ml 100% ethanol; 250 micrograms PGE2/0.2 ml 100% ethanol; 250 micrograms acetazolamide/0.2 ml 100% ethanol; 250 micrograms PGE1 or PGE2 + 250 micrograms acetazolamide/0.2 ml 100% ethanol. These solutions were given in a single bolus on day 6 postinoculation (PI) or twice daily on days 6-9 PI. Following these treatments the number of parasite ova per gram feces per day for days 6-10 PI and numbers of worms present at necropsy on day 10 PI were determined. Treatment with prostaglandins or acetazolamide or both failed to adversely affect egg deposition by adult female worms or the number of worms in the small intestine. These results do not support the involvement of prostaglandins in the expulsion of N. brasiliensis from the host intestine.
据报道，前列腺素E1（PGE1）和前列腺素E2（PGE2）可促进巴西日圆线虫（一种线虫寄生虫）从大鼠肠道自然排出。黏液分泌可能也是排虫的关键因素。我们的研究试图确定：1）PGE1或PGE2是否会改变大鼠感染巴西日圆线虫的正常病程；2）一种已知的黏液增强药物乙酰唑胺是否能提高排虫率；3）前列腺素与乙酰唑胺联合使用是否会对大鼠体内的巴西日圆线虫产生影响。给大鼠接种约1000条巴西日圆线虫感染性幼虫。通过十二指肠内给药，给予动物以下其中一种：0.2毫升0.9%氯化钠溶液；0.2毫升无水乙醇；250微克PGE1/0.2毫升无水乙醇；250微克PGE2/0.2毫升无水乙醇；250微克乙酰唑胺/0.2毫升无水乙醇；250微克PGE1或PGE2 + 250微克乙酰唑胺/0.2毫升无水乙醇。这些溶液在接种后第6天单次推注给药，或在接种后第6 - 9天每日给药两次。在这些处理后，测定接种后第6 - 10天每天每克粪便中的寄生虫卵数量，以及接种后第10天尸检时的虫体数量。用前列腺素或乙酰唑胺或两者联合处理均未对成年雌虫的排卵或小肠内的虫体数量产生不利影响。这些结果不支持前列腺素参与巴西日圆线虫从宿主体内排出的过程。
