Peek M J, Norman T M, Morgan C, Fraser I S, Markham R
Department of Obstetrics and Gynaecology, University of Sydney, New South Wales, Australia.
Prostaglandins. 1987 Dec;34(6):919-25. doi: 10.1016/0090-6980(87)90071-2.
Controversy still continues concerning the factors controlling endometrial prostaglandin concentrations measured under different circumstances. In this study an attempt was made to remove serial samples of normal human endometrium and standardise 4 different short incubation periods at room temperature before snap-freezing the tissue. No significant difference was found in the concentrations of PGE2 and PGF2 alpha, or PGE2/PGF2 alpha ratio, of the series of specimens with the shortest incubation (42.7 seconds) compared with the longest (93.1 secs). The most likely explanation for this is that trauma-induced prostaglandin production is such a rapid process that it is generally complete within less than 30 seconds at room temperature. As reported in previous studies, a significant correlation was found between PGE2 and PGF2 alpha concentrations in some specimens. Large and significant variations in concentration of prostaglandins were found between individuals which were partly accounted for by variations in stage of the menstrual cycle at the time of collection.
关于在不同情况下测量子宫内膜前列腺素浓度的控制因素，争议仍在继续。在本研究中，尝试采集正常人子宫内膜的系列样本，并在室温下对4种不同的短孵育期进行标准化，然后将组织速冻。与最长孵育期（93.1秒）的系列标本相比，最短孵育期（42.7秒）的系列标本中PGE2和PGF2α的浓度或PGE2/PGF2α比值没有显著差异。对此最可能的解释是，创伤诱导的前列腺素产生是一个快速过程，在室温下通常在不到30秒内就完成了。如先前研究报道，在一些标本中发现PGE2和PGF2α浓度之间存在显著相关性。个体之间前列腺素浓度存在巨大且显著的差异，部分原因是采集时月经周期阶段的变化。
