Peek M J, Fraser I S, Phillips C A, Resta T M, Blackwell P M, Markham R
Prostaglandins. 1985 Jan;29(1):3-18. doi: 10.1016/0090-6980(85)90146-7.
Two in vitro methods for measuring human endometrial prostaglandin production were compared. Endometrial samples from eight patients were incubated over eight hours by a perifusion and a superfusion technique. The collected fractions were assayed by radioimmunoassay for PGE2 and PGF2 alpha. There was no significant difference between the perifusion and superfusion methods for the pattern and amount of PGE2 and PGF2 alpha production with time. Significantly higher production levels of PGE2 and PGF2 alpha were found in secretory phase endometria than in proliferative phase endometria. Histological examination of the tissue specimens by light and electron microscopy showed that both methods caused gross tissue damage after eight hours experimentation. The superfusion method produced more morphological damage than the perifusion method. However, no tissue damage could be detected after one hour of incubation with either method. Over an eight hour period neither the perifusion nor the superfusion technique appears to be a good indicator of in vivo endometrial prostaglandin production. Either technique used for only one to two hours may better reflect the in vivo situation.
比较了两种测量人子宫内膜前列腺素生成的体外方法。采用灌流和超灌流技术，对8例患者的子宫内膜样本进行了8小时的孵育。收集的组分通过放射免疫分析法测定PGE2和PGF2α。就PGE2和PGF2α生成的模式和量随时间的变化而言，灌流法和超灌流法之间没有显著差异。分泌期子宫内膜中PGE2和PGF2α的生成水平显著高于增殖期子宫内膜。通过光学显微镜和电子显微镜对组织标本进行组织学检查显示，两种方法在8小时实验后均造成了明显的组织损伤。超灌流法造成的形态学损伤比灌流法更严重。然而，两种方法孵育1小时后均未检测到组织损伤。在8小时期间，灌流技术和超灌流技术似乎都不是体内子宫内膜前列腺素生成的良好指标。仅使用1至2小时的任何一种技术可能更能反映体内情况。
