Ishikawa Eiji, Yamada Tetsuya, Yamaji Kazuaki, Serata Masaki, Fujii Daichi, Umesaki Yoshinori, Tsuji Hirokazu, Nomoto Koji, Ito Masahiro, Okada Nobuhiko, Nagaoka Masato, Gomi Atsushi
Yakult Central Institute, 5-11 Izumi, Kunitachi-shi, Tokyo 186-8650, Japan.
Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
iScience. 2021 Oct 28;24(11):103363. doi: 10.1016/j.isci.2021.103363. eCollection 2021 Nov 19.
YIT 10347 (BF-1) is adhesive . Here we studied the molecular aspects of the BF-1 adhesion process. We identified and characterized non-adhesive mutants and found that a class E housekeeping sortase was critical for the adhesion to mucin. These mutants were significantly less adhesive to GCIY cells than was the wild type (WT), which protected GCIY cells against acid treatment more than did a non-adhesive mutant. The non-adhesive mutants aberrantly accumulated precursors of putative sortase-dependent proteins (SDPs). Recombinant SDPs bound to mucin. Disruption of the housekeeping sortase influenced expression of SDPs and pilus components. Mutants defective in a pilin or in an SDP showed the same adhesion properties as WT. Therefore, multiple SDPs and pili seem to work cooperatively to achieve adhesion, and the housekeeping sortase is responsible for cell wall anchoring of its substrates to ensure their proper biological function.
YIT 10347（BF - 1）具有黏附性。在此，我们研究了BF - 1黏附过程的分子层面。我们鉴定并表征了非黏附性突变体，发现一类E型管家分选酶对黏附黏蛋白至关重要。这些突变体对GCIY细胞的黏附性明显低于野生型（WT），与非黏附性突变体相比，野生型对GCIY细胞的酸处理保护作用更强。非黏附性突变体异常积累了假定的分选酶依赖性蛋白（SDPs）的前体。重组SDPs与黏蛋白结合。管家分选酶的破坏影响了SDPs和菌毛成分的表达。菌毛蛋白或SDP有缺陷的突变体表现出与野生型相同的黏附特性。因此，多种SDPs和菌毛似乎协同作用以实现黏附，而管家分选酶负责将其底物锚定在细胞壁上，以确保其正常的生物学功能。
