Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, Guizhou University, Guiyang City, 550025, Guizhou, China.
Anal Bioanal Chem. 2022 Feb;414(4):1461-1468. doi: 10.1007/s00216-021-03799-2. Epub 2021 Nov 25.
Surface-enhanced Raman spectroscopy (SERS), as a label-free, highly sensitive analytical method, has become an important tool for providing substance fingerprints. In this study, silver nanoparticles containing thiosulfate ions and calcium ions (Ag@SCNPs) have been used as an enhanced substrate to eliminate the interference of impurities on DNA signals. Intrinsic structural information on single-strand DNA (ssDNA) was directly obtained through SERS. The improved enhancement system was used to explore the base-stacking rules of ssDNA in a solution environment. For the first time, single-base insertion mutations and deletion mutations, as well as their exact mutation sites, were identified, and Raman spectra with high stability, repeatability, and high signal-to-noise ratio were obtained. The method is simple, fast, and accurate, and the detection process is nondestructive. It has potential to be applied in the fields of medical diagnosis and genetics research.
表面增强拉曼光谱（SERS）作为一种无标记、高灵敏度的分析方法，已成为提供物质指纹图谱的重要工具。在这项研究中，含有硫代硫酸根离子和钙离子的银纳米粒子（Ag@SCNPs）被用作增强基底，以消除杂质对 DNA 信号的干扰。通过 SERS 直接获得了单链 DNA（ssDNA）的固有结构信息。改进的增强系统用于在溶液环境中研究 ssDNA 的碱基堆积规则。首次识别了单碱基插入突变和缺失突变，以及它们的确切突变位点，并获得了具有高稳定性、重复性和高信噪比的拉曼光谱。该方法简单、快速、准确，检测过程无损。它有可能应用于医学诊断和遗传学研究领域。
