College of Chemistry and Chemical Engineering, Guizhou University, No. 2708, South Section of Huaxi Avenue, Guiyang City, Guizhou Province 550025, China.
School of Physics, Guizhou University, No. 2708, South Section of Huaxi Avenue, Guiyang City, Guizhou Province 550025, China.
Anal Chem. 2021 Feb 2;93(4):1951-1956. doi: 10.1021/acs.analchem.0c04052. Epub 2021 Jan 19.
Recent developments in molecular spectroscopy have widened the scope of surface-enhanced Raman spectroscopy (SERS) for detection of nucleic acids. In order to solve the interference of impurity signals in SERS analysis that hamper the reliable detection of DNA, Ag nanoparticles modified with thiosulfate ions were used to obtain SERS signals of DNA molecules in aqueous solutions, which showed good reproducibility. By using thiosulfate ions and calcium ions as aggregating agents, this method not only eliminated the influence of citrate on DNA signals completely but also obtained the signals for all bases indiscriminately, including the T base that was considered to have low Raman activity. Subsequently, the base stacking rule was used to identify mutations arising from C/T transition. It further identified the mutation sites of single-base C/T transition using this platform for the first time. This method has wide application prospects in DNA analysis, DNA sequencing, and genetic testing.
近年来，分子光谱学的发展拓宽了表面增强拉曼光谱（SERS）在检测核酸方面的应用范围。为了解决 SERS 分析中杂质信号干扰阻碍 DNA 可靠检测的问题，采用巯基乙磺酸离子修饰的银纳米粒子在水溶液中获得了 DNA 分子的 SERS 信号，该方法具有良好的重现性。通过使用硫代硫酸根离子和钙离子作为聚集剂，该方法不仅完全消除了柠檬酸盐对 DNA 信号的影响，而且还能不分碱基地获得所有碱基的信号，包括被认为拉曼活性低的 T 碱基。随后，基于碱基堆积规则对由 C/T 转换引起的突变进行了识别。该方法首次在此平台上用于鉴定单碱基 C/T 转换的突变位点。该方法在 DNA 分析、DNA 测序和基因检测方面具有广泛的应用前景。
