通过工程化的游动放线菌（Nonomuraea gerenzanensis）菌株提高糖肽抗生素 A40926 的产量。
Production enhancement of the glycopeptide antibiotic A40926 by an engineered Nonomuraea gerenzanensis strain.
机构信息
School of Pharmacy, Liaocheng University, No. 1, Hunan road, Dongchangfu District, Liaocheng, 252000, Shandong, China.
出版信息
Biotechnol Lett. 2022 Feb;44(2):259-269. doi: 10.1007/s10529-021-03210-1. Epub 2021 Nov 26.
OBJECTIVE
To improve the production of A40926, a combined strategy of constructing the engineered strain and optimizing the medium was implemented.
RESULTS
The engineered strain lcu1 with the genetic features of dbv23 deletion and dbv3-dbv20 coexpression increased by 30.6% in the production of A40926, compared to the original strain. In addition, a combined medium called M9 was designed to be further optimized by the central composite design method. The optimized M9 medium was verified to significantly improve the A40926 yield from 257 to 332 mg l.
CONCLUSIONS
The engineered strain lcu1 could significantly promote A40926 production in the optimized M9 medium, which indicated that the polygenic genetic manipulation and the media optimization played an equally important role in increasing the A40926 yield.
目的
通过构建工程菌株和优化培养基的联合策略，提高 A40926 的产量。
结果
与原始菌株相比，具有 dbv23 缺失和 dbv3-dbv20 共表达遗传特征的工程菌株 lcu1 使 A40926 的产量提高了 30.6%。此外，设计了一种名为 M9 的组合培养基，并通过中心复合设计方法进一步进行优化。验证表明，优化的 M9 培养基可使 A40926 的产量从 257mg/L 显著提高至 332mg/L。
结论
工程菌株 lcu1 可在优化的 M9 培养基中显著促进 A40926 的生产，这表明多基因遗传操作和培养基优化在提高 A40926 产量方面具有同等重要的作用。
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