State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing, 210023, China.
State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing, 210023, China.
Chemosphere. 2022 Mar;291(Pt 1):133004. doi: 10.1016/j.chemosphere.2021.133004. Epub 2021 Nov 24.
Molybdenum disulfide (MoS) is a transition metal dichalcogenides (TMDCs) material that is seeing rapidly increasing use. The wide range of applications will result in significant environmental release. Here, the impact of MoS nanosheets on rice and associated soil microbial communities was evaluated. Rice plants were grown for 4 weeks in a natural paddy soil amended with either 1T or 2H phase MoS nanosheets at 10 and 100 mg kg. The 1T MoS nanosheets have a significantly greater dissolution rate (58.9%) compared to 2H MoS (4.4%), indicating the instability of 1T MoS in environment. High dissolution rate resulted in a high Mo bioaccumulation in rice leaves (272 and 189 mg kg under 1T and 2H exposure at 100 mg kg). However, this did not induce overt phytotoxicity, as indicated by a range of phenotypic or biochemical based determine endpoints, e.g., biomass, photosynthetic pigments, and malondialdehyde (MDA) content. Additionally, rice P uptake was significantly increased upon exposure to 1T and 2H MoS (10 mg kg). Gas chromatography-mass spectrometry (GC-MS) reveals that both phases of MoS in soil systematically enhanced the carbon and nitrogen related metabolic pathways in exposed plants. Soil 16S rRNA gene sequencing data show that soil microbial community structure was unchanged upon MoS exposure. However, both phases of MoS remarkably increased the relative abundance of N-fixation cyanobacteria, and 2H MoS exposure increased a plant growth-promoting rhizobacteria-Bacillus. Overall, our results suggest that MoS nanosheets at tested doses did not exert negative impacts on rice plant and the associated soil microbial community.
二硫化钼（MoS）是一种过渡金属二硫属化物（TMDCs）材料，其应用越来越广泛，因此预计会大量释放到环境中。本研究评价了 MoS 纳米片对水稻及其相关土壤微生物群落的影响。将水稻植株在添加了 1T 或 2H 相 MoS 纳米片的天然稻田土壤中培养 4 周，浓度分别为 10 和 100mg/kg。与 2H MoS（4.4%）相比，1T MoS 纳米片的溶解速率（58.9%）明显更高，表明 1T MoS 在环境中不稳定。高溶解速率导致水稻叶片中的 Mo 生物累积量很高（100mg/kg 时，1T 和 2H 暴露下分别为 272 和 189mg/kg）。然而，这并没有引起明显的植物毒性，因为一系列表型或基于生化的终点（例如生物量、光合色素和丙二醛（MDA）含量）都没有显示出毒性。此外，水稻对 1T 和 2H MoS（10mg/kg）的吸收显著增加。气相色谱-质谱联用（GC-MS）表明，土壤中 MoS 的两种相都系统地增强了暴露植物中与碳和氮相关的代谢途径。土壤 16S rRNA 基因测序数据表明，MoS 暴露并未改变土壤微生物群落结构。然而，MoS 的两种相都显著增加了固氮蓝藻的相对丰度，而 2H MoS 暴露增加了植物促生根瘤菌-芽孢杆菌的相对丰度。总的来说，我们的结果表明，在测试剂量下，MoS 纳米片对水稻植株及其相关土壤微生物群落没有产生负面影响。
