Nanoscience Graduate Program, Universidade Franciscana, Santa Maria, RS, Brazil.
Polytechnical School of Chemical Engineering, Universidade de São Paulo, São Paulo, SP, Brazil.
J Biotechnol. 2022 Jan 10;343:47-51. doi: 10.1016/j.jbiotec.2021.11.003. Epub 2021 Nov 23.
The industrial effluent contaminated with organic pollutants has been causing an increase in the toxicity of the ecosystem, causing a great environmental impact. Thus, the present work aims the green synthesis of silver nanoparticles (AgNPs) from Aloe vera, its characterization and antimicrobial activity against Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923). AgNPs were characterized by X-ray diffraction (XRD), Scanning Electronic Microscopy with Energy Dispersive Spectroscopy (SEM-EDS), Zeta Potential (ZP) and N porosimetry (BET/BJH method). Antimicrobial activity were carried out by Minimal Inhibitory Concentration (MIC) method. The XRD demonstrated characteristic peaks of AgNPs at 38.29°; 44.55° and 64.81°, and SEM-EDS micrographs showed that AgNPs produced by biomolecules of Aloe vera extract resulted in a weight concentration around 92.59% silver, 7.15% oxygen and 0.26% chlorine. Regarding zeta potential, all samples showed negative electric charge (around -35.3 mV), while N porosimetry resulted in a surface specific area of 6.09 m g, with a volume and diameter pore of 0.032 cm³ g and 33.47, respectively. Antimicrobial activity was observed at 15.62 µg mL and 31.25 µg mL for P. aeruginosa and S. aureus, respectively. Thus, AgNPs can be considered a promising nanoparticle for degradation of organic pollutants in aqueous solution as well as an adjuvant for treatment of microbial infections.
工业废水中的有机污染物导致生态系统毒性增加,对环境造成了巨大影响。因此,本工作旨在从芦荟中绿色合成银纳米粒子 (AgNPs),并对其进行表征以及对铜绿假单胞菌 (ATCC 27853) 和金黄色葡萄球菌 (ATCC 25923) 的抗菌活性进行研究。AgNPs 的特征通过 X 射线衍射 (XRD)、带有能量色散光谱 (SEM-EDS) 的扫描电子显微镜、Zeta 电位 (ZP) 和 N 孔隙率 (BET/BJH 法) 进行表征。抗菌活性通过最小抑菌浓度 (MIC) 法进行测定。XRD 显示 AgNPs 的特征峰在 38.29°、44.55°和 64.81°,SEM-EDS 显微照片显示,由芦荟提取物的生物分子产生的 AgNPs 的重量浓度约为 92.59%银、7.15%氧和 0.26%氯。关于 Zeta 电位,所有样品均显示负电荷(约 -35.3 mV),而 N 孔隙率导致比表面积为 6.09 m g,孔体积和直径分别为 0.032 cm³ g 和 33.47。对铜绿假单胞菌和金黄色葡萄球菌的抗菌活性分别在 15.62 µg mL 和 31.25 µg mL 时观察到。因此,AgNPs 可以被认为是一种有前途的纳米粒子,可用于降解水溶液中的有机污染物,也可作为治疗微生物感染的辅助剂。