利用蔓越莓粉水提取物绿色合成银纳米颗粒：表征及抗菌性能
Green synthesis of silver nanoparticles using cranberry powder aqueous extract: characterization and antimicrobial properties.
作者信息
Ashour Asmaa A, Raafat Dina, El-Gowelli Hanan M, El-Kamel Amal H
机构信息
Department of Pharmaceutics, Alexandria University, Alexandria, Egypt.
Department of Pharmaceutical Microbiology, Alexandria University, Alexandria, Egypt.
出版信息
Int J Nanomedicine. 2015 Dec 1;10:7207-21. doi: 10.2147/IJN.S87268. eCollection 2015.
BACKGROUND
The growing threat of microbial resistance against traditional antibiotics has prompted the development of several antimicrobial nanoparticles (NPs), including silver NPs (AgNPs). In this article, a simple and eco-friendly method for the synthesis of AgNPs using the cranberry powder aqueous extract is reported.
MATERIALS AND METHODS
Cranberry powder aqueous extracts (0.2%, 0.5%, and 0.8% w/v) were allowed to interact for 24 hours with a silver nitrate solution (10 mM) at 30°C at a ratio of 1:10. The formation of AgNPs was confirmed by ultraviolet-visible spectroscopy and their concentrations were determined using atomic absorption spectroscopy. The prepared NPs were evaluated by transmission electron microscopy, measurement of ζ-potential, and Fourier-transform infrared spectroscopy. The in vitro antimicrobial properties of AgNPs were then investigated against several microbial strains. Finally, in vivo appraisal of both wound-healing and antimicrobial properties of either plain AgNPs (prepared using 0.2% extract) or AgNP-Pluronic F-127 gel was conducted in a rat model after induction of a Staphylococcus aureus ATCC 6538P wound infection.
RESULTS
The formation of AgNPs was confirmed by ultraviolet-visible spectroscopy, where a surface-plasmon resonance absorption peak was observed between 432 and 438 nm. Both size and concentration of the formed AgNPs increased with increasing concentration of the extracts. The developed NPs were stable, almost spherical, and polydisperse, with a size range of 1.4-8.6 nm. The negative ζ-potential values, as well as Fourier-transform infrared spectroscopy analysis, indicated the presence of a capping agent adsorbed onto the surface of the particles. In vitro antimicrobial evaluation revealed a size-dependent activity of the AgNPs against the tested organisms. Finally, AgNPs prepared using 0.2% extract exhibited a substantial in vivo healing potential for full-thickness excision wounds in rats.
CONCLUSION
AgNPs were successfully synthesized from a silver nitrate solution through a simple green route, using cranberry powder aqueous extract as a reducing as well as capping agent.
背景
微生物对传统抗生素耐药性的威胁日益增加，促使人们开发了几种抗菌纳米颗粒（NPs），包括银纳米颗粒（AgNPs）。本文报道了一种使用蔓越莓粉水提取物合成AgNPs的简单且环保的方法。
材料与方法
将蔓越莓粉水提取物（0.2%、0.5%和0.8%，w/v）与硝酸银溶液（10 mM）在30℃下以1:10的比例相互作用24小时。通过紫外可见光谱确认AgNPs的形成，并使用原子吸收光谱法测定其浓度。通过透射电子显微镜、ζ电位测量和傅里叶变换红外光谱对制备的NPs进行评估。然后研究了AgNPs对几种微生物菌株的体外抗菌性能。最后，在诱导金黄色葡萄球菌ATCC 6538P伤口感染的大鼠模型中，对普通AgNPs（使用0.2%提取物制备）或AgNP-普朗尼克F-127凝胶的伤口愈合和抗菌性能进行了体内评估。
结果
通过紫外可见光谱确认了AgNPs的形成，在432至438 nm之间观察到表面等离子体共振吸收峰。随着提取物浓度的增加，形成的AgNPs的尺寸和浓度均增加。所制备的NPs稳定，几乎呈球形且多分散，尺寸范围为1.4-8.6 nm。负ζ电位值以及傅里叶变换红外光谱分析表明颗粒表面存在封端剂。体外抗菌评估显示AgNPs对受试生物具有尺寸依赖性活性。最后，使用0.2%提取物制备的AgNPs对大鼠全层切除伤口具有显著的体内愈合潜力。
结论
以蔓越莓粉水提取物作为还原剂和封端剂，通过简单的绿色途径成功地从硝酸银溶液中合成了AgNPs。
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