Nguyen Thi Thanh Hanh, Lee Dong-Gu, Apu Md Aminul Islam, Jung Jong-Hyun, Kim Min-Kyu, Lim Sangyong, Chung Byoungsang, Pal Kunal, Kim Doman
Institute of Food Industrialization, Institutes of Green Bio Science & Technology, Seoul National University, Pyeongchang-gun, 25354, Gangwon-do, Republic of Korea.
Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang-gun, 25354, Gangwon-do, Republic of Korea.
Enzyme Microb Technol. 2022 Jan;153:109955. doi: 10.1016/j.enzmictec.2021.109955. Epub 2021 Nov 20.
Non-digestible isomaltooligosaccharides (NDIMOS) are functional food and beverage ingredients that contributed to human health benefits through metabolism of gastrointestinal microorganism. In this study, NDIMOS were synthesized by combine dextransucrase from Leuconostoc mesenteroides B512F/KM and alternansucrase from L. mesenteroides NRRL 1355CF10/KM using sucrose as substrate and maltose as acceptor. Their digestibility was confirmed by using digestive enzymes including α-amylase and amyloglucosidase. NDIMOS inhibited insoluble glucan formation through mutansucrase from Streptococcus mutans. The bifidogenic effect of NDIMOS was investigated by growth of four strains of Bifidobacterium in MRS broth containing NDIMOS, compared with MRS broth contain glucose and negative control. Additionally, Bifidobacterium bifidum or Bifidobacterium adolescentis inhibited the growth of Salmonella enterica serovar typhimurium when they were co-cultivation in MRS broth containing NDIMOS. These results suggested that NDIMOS is potential functional ingredient for food, beverage, and pharmaceutical application.
非消化性异麦芽低聚糖（NDIMOS）是功能性食品和饮料成分，可通过胃肠道微生物的代谢对人体健康有益。在本研究中，以蔗糖为底物、麦芽糖为受体，将来自肠系膜明串珠菌B512F/KM的葡聚糖蔗糖酶和来自肠系膜明串珠菌NRRL 1355CF10/KM的交替蔗糖酶结合，合成了NDIMOS。通过使用包括α-淀粉酶和淀粉葡糖苷酶在内的消化酶证实了它们的消化性。NDIMOS通过变形链球菌的变蔗糖酶抑制不溶性葡聚糖的形成。通过在含有NDIMOS的MRS肉汤中培养四株双歧杆菌，与含有葡萄糖的MRS肉汤和阴性对照相比，研究了NDIMOS的双歧增殖作用。此外，当双歧双歧杆菌或青春双歧杆菌在含有NDIMOS的MRS肉汤中共同培养时，它们会抑制鼠伤寒沙门氏菌血清型鼠伤寒杆菌的生长。这些结果表明，NDIMOS是食品、饮料和制药应用中的潜在功能性成分。
