Kanygin Vladimir, Razumov Ivan, Zaboronok Alexander, Zavjalov Evgenii, Kichigin Aleksandr, Solovieva Olga, Tsygankova Alphiya, Guselnikova Tatiana, Kasatov Dmitrii, Sycheva Tatiana, Mathis Bryan J, Taskaev Sergey
Laboratory of Medical and Biological Problems of BNCT, Novosibirsk State University, 1 Pirogov Str., 630090 Novosibirsk, Russia.
Center for Genetic Resources of Laboratory Animals, Institute of Cytology and Genetics SB RAS, 10, Acad. Lavrentieva Ave., 630090 Novosibirsk, Russia.
Biology (Basel). 2021 Nov 2;10(11):1124. doi: 10.3390/biology10111124.
(1) Background: Developments in accelerator-based neutron sources moved boron neutron capture therapy (BNCT) to the next phase, where new neutron radiation parameters had to be studied for the treatment of cancers, including brain tumors. We aimed to further improve accelerator-BNCT efficacy by optimizing dosimetry control, beam parameters, and combinations of boronophenylalanine (BPA) and sodium borocaptate (BSH) administration in U87MG xenograft-bearing immunodeficient mice with two different tumor locations. (2) Methods: The study included two sets of experiments. In Experiment #1, BPA only and single or double irradiation in higher doses were used, while, in Experiment #2, BPA and BSH combinations and single or double irradiation with dosage adjustment were analyzed. Mice without treatment or irradiation after BPA or BPA+BSH injection were used as controls. (3) Results: Irradiation parameter adjustment and BPA and BSH combination led to 80-83% tumor-growth inhibition index scores, irradiation:BNCT ratios of 1:2, and increases in animal life expectancy from 9 to 107 days. (4) Conclusions: Adjustments in dosimetry control, calculation of irradiation doses, and combined use of two B compounds allowed for BNCT optimization that will be useful in the development of clinical-trial protocols for accelerator-based BNCT.
(1) 背景：基于加速器的中子源的发展使硼中子俘获疗法（BNCT）进入了下一阶段，在此阶段必须研究新的中子辐射参数以用于癌症治疗，包括脑肿瘤。我们旨在通过优化剂量控制、束流参数以及在两种不同肿瘤位置的携带U87MG异种移植瘤的免疫缺陷小鼠中硼苯丙氨酸（BPA）和硼酸钠（BSH）给药的组合，进一步提高加速器BNCT的疗效。(2) 方法：该研究包括两组实验。在实验#1中，仅使用BPA以及更高剂量的单次或两次照射，而在实验#2中，分析了BPA和BSH的组合以及调整剂量后的单次或两次照射。在注射BPA或BPA + BSH后未进行治疗或照射的小鼠用作对照。(3) 结果：照射参数调整以及BPA和BSH的组合导致肿瘤生长抑制指数得分达到80 - 83%，照射：BNCT比率为1:2，并且动物预期寿命从9天增加到107天。(4) 结论：剂量控制的调整、照射剂量的计算以及两种硼化合物的联合使用实现了BNCT的优化，这将有助于基于加速器的BNCT临床试验方案的制定。
