Greiner Jack V, Glonek Thomas
The Schepens Eye Research Institute of Massachusetts Eye & Ear Infirmary, Boston, MA 02114, USA.
Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.
Biology (Basel). 2021 Nov 12;10(11):1166. doi: 10.3390/biology10111166.
Crystalline lens and striated muscle exist at opposite ends of the metabolic spectrum. Lens is a metabolically quiescent tissue, whereas striated muscle is a mechanically dynamic tissue with high-energy requirements, yet both tissues contain millimolar levels of ATP (>2.3 mM), far exceeding their underlying metabolic needs. We explored intracellular concentrations of ATP across multiple cells, tissues, species, and domains to provide context for interpreting lens/striated muscle data. Our database revealed that high intracellular ATP concentrations are ubiquitous across diverse life forms including species existing from the Precambrian Era, suggesting an ancient highly conserved role for ATP, independent of its widely accepted view as primarily "metabolic currency". Our findings reinforce suggestions that the primordial function of ATP was non-metabolic in nature, serving instead to prevent protein aggregation.
晶状体和横纹肌处于新陈代谢谱的两端。晶状体是一种代谢静止的组织，而横纹肌是一种具有高能量需求的机械动态组织，但这两种组织都含有毫摩尔水平的三磷酸腺苷（>2.3 mM），远远超过其潜在的代谢需求。我们探索了多种细胞、组织、物种和生物域中的三磷酸腺苷细胞内浓度，以为解释晶状体/横纹肌数据提供背景。我们的数据库显示，高细胞内三磷酸腺苷浓度在包括前寒武纪时代就已存在的物种在内的各种生命形式中普遍存在，这表明三磷酸腺苷具有古老的高度保守作用，与其被广泛接受的主要作为“代谢货币”的观点无关。我们的研究结果强化了这样的观点，即三磷酸腺苷的原始功能本质上是非代谢性的，而是用于防止蛋白质聚集。
