Querido Micaela Machado, Paulo Ivo, Hariharakrishnan Sriram, Rocha Daniel, Barbosa Nuno, Galhano Dos Santos Rui, Bordado João Moura, Teixeira João Paulo, Pereira Cristiana Costa
Environmental Health Department, National Institute of Health, 4000-055 Porto, Portugal.
EPIUnit, Institute of Public Health, University of Porto, 4050-600 Porto, Portugal.
Antibiotics (Basel). 2021 Nov 5;10(11):1351. doi: 10.3390/antibiotics10111351.
The risk of infection arising from indirect sources-namely, contaminated surfaces-has been proved, particularly in healthcare facilities. In the attempt to minimize this problem, innumerable research projects involving the development of surfaces with self-disinfecting properties are being conducted. In this work, wall-paints with self-disinfecting properties were developed with the scope of being applied in environments prone to contamination, such as those at healthcare settings. Our approach was to develop new paint formulations containing two natural plant-based products with known antimicrobial activity-colophony (CLF) and curcumin (CUR). The natural substances were separately incorporated on a commercial paint and their antibacterial activity was evaluated with several bacterial species following ISO 22196. To assess the paints' safety, cytotoxicity tests were performed on HaCaT and A549 cell lines, using tests on extracts and direct contact tests, as suggested by the standardized protocol ISO 10993. In general, both paints containing CLF and CUR were able to reduce the bacterial growth after 24 h, compared with the control, the commercial unmodified paint. Colophony was even able to reduce the number of culturable bacteria by over 2 log for , , and . Regarding the cytotoxicity tests performed (WST-1, NRU, and LDH), both formulations revealed promising results regardless of the methodology used.
已证实间接来源（即受污染的表面）会引发感染风险，在医疗保健机构中尤为如此。为尽量减少这一问题，目前正在开展无数涉及开发具有自消毒特性表面的研究项目。在这项工作中，开发了具有自消毒特性的墙面漆，旨在应用于易受污染的环境，如医疗保健场所。我们的方法是开发新的涂料配方，其中包含两种具有已知抗菌活性的天然植物产品——松香（CLF）和姜黄素（CUR）。将这些天然物质分别添加到一种商用涂料中，并按照ISO 22196对几种细菌进行抗菌活性评估。为评估这些涂料的安全性，按照标准化协议ISO 10993的建议，对HaCaT和A549细胞系进行了细胞毒性测试，包括提取物测试和直接接触测试。总体而言，与对照（未改性的商用涂料）相比，含有CLF和CUR的两种涂料在24小时后均能抑制细菌生长。松香甚至能使金黄色葡萄球菌、大肠杆菌和白色念珠菌的可培养细菌数量减少超过2个对数级。关于所进行的细胞毒性测试（WST-1、NRU和LDH），无论采用何种方法，两种配方均显示出良好的结果。
