Silvia Sophia, Donahue Samantha A, Killeavy Erin E, Jogl Gerwald, Gregory Steven T
Department of Cell and Molecular Biology, The University of Rhode Island, Kingston, RI 02881, USA.
Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA.
Antibiotics (Basel). 2021 Nov 11;10(11):1384. doi: 10.3390/antibiotics10111384.
is a halophilic extreme thermophile, with potential as a model organism for studies of the structural basis of antibiotic resistance. In order to facilitate genetic studies of this organism, we have surveyed the antibiotic sensitivity spectrum of and identified spontaneous antibiotic-resistant mutants. is naturally insensitive to aminoglycosides, aminocylitols and tuberactinomycins that target the 30S ribosomal subunit, but is sensitive to all 50S ribosomal subunit-targeting antibiotics examined, including macrolides, lincosamides, streptogramin B, chloramphenicol, and thiostrepton. It is also sensitive to kirromycin and fusidic acid, which target protein synthesis factors. It is sensitive to rifampicin (RNA polymerase inhibitor) and to the fluoroquinolones ofloxacin and ciprofloxacin (DNA gyrase inhibitors), but insensitive to nalidixic acid. Drug-resistant mutants were identified using rifampicin, thiostrepton, erythromycin, spiramycin, tylosin, lincomycin, and chloramphenicol. The majority of these were found to have mutations that are similar or identical to those previously found in other species, while several novel mutations were identified. This study provides potential selectable markers for genetic manipulations and demonstrates the feasibility of using as a model system for studies of ribosome and RNA polymerase structure, function, and evolution.
是一种嗜盐极端嗜热菌，有潜力作为研究抗生素抗性结构基础的模式生物。为了便于对该生物体进行遗传学研究，我们调查了其抗生素敏感性谱并鉴定出自发的抗生素抗性突变体。对靶向30S核糖体亚基的氨基糖苷类、氨基环醇类和结核放线菌素天然不敏感，但对所检测的所有靶向50S核糖体亚基的抗生素敏感，包括大环内酯类、林可酰胺类、链阳霉素B、氯霉素和硫链丝菌素。它对靶向蛋白质合成因子的奇霉素和夫西地酸也敏感。它对利福平（RNA聚合酶抑制剂）以及氟喹诺酮类药物氧氟沙星和环丙沙星（DNA回旋酶抑制剂）敏感，但对萘啶酸不敏感。使用利福平、硫链丝菌素、红霉素、螺旋霉素、泰乐菌素、林可霉素和氯霉素鉴定出耐药突变体。发现其中大多数具有与其他物种中先前发现的突变相似或相同的突变，同时鉴定出了几个新的突变。本研究为基因操作提供了潜在的选择标记，并证明了将其用作研究核糖体和RNA聚合酶结构、功能及进化的模式系统的可行性。
