Argüelles Alejandra, Sánchez-Fresneda Ruth, Martínez-Mármol Elisa, Lozano José Antonio, Solano Francisco, Argüelles Juan Carlos
Vitalgaia, España, S.L., 30005 Murcia, Spain.
Área de Microbiología, Facultad de Biología, Universidad de Murcia, 30071 Murcia, Spain.
Antibiotics (Basel). 2021 Nov 13;10(11):1395. doi: 10.3390/antibiotics10111395.
Current antifungal chemotherapy against the prevalent basidiomycete displays some drawbacks. This pathogenic fungus is refractory to echinocandins, whereas conventional treatment with amphotericin B plus 5-fluorocytosine has a limited efficacy. In this study, we explored the potential cryptococcal activity of some natural agents. After conducting a screening test with a set of propolis from different geographical areas, we selected an extract from China, which displayed a certain cytotoxic activity against , due to this extract being cheap and easily available in large amounts. The combination of this kind of propolis with carnosic acid in a 1:4 ratio induced a stronger fungicidal effect, which occurred following a synergistic pattern, without visible alterations in external cell morphology. Furthermore, several carnosic acid-propolis formulations applied onto preformed biofilms decreased the metabolic activity of the sessile cells forming biofilms. These data support the potential application of mixtures containing these two natural extracts in the design of new antifungal strategies in order to combat opportunistic infections caused by prevalent pathogenic fungi.
目前针对常见担子菌的抗真菌化疗存在一些缺点。这种致病真菌对棘白菌素耐药，而两性霉素B加5-氟胞嘧啶的传统治疗效果有限。在本研究中，我们探索了一些天然药物对新型隐球菌的潜在活性。在用来自不同地理区域的一组蜂胶进行筛选试验后，我们选择了一种来自中国的提取物，由于该提取物价格低廉且易于大量获取，它对新型隐球菌表现出一定的细胞毒性活性。这种蜂胶与鼠尾草酸以1:4的比例组合可诱导更强的杀菌效果，这种效果呈协同模式出现，且细胞外部形态无明显改变。此外，几种应用于预先形成的生物膜上的鼠尾草酸 - 蜂胶制剂降低了形成生物膜的固着细胞的代谢活性。这些数据支持了含有这两种天然提取物的混合物在设计新的抗真菌策略以对抗由常见致病真菌引起的机会性感染方面的潜在应用。
