Gucwa Katarzyna, Kusznierewicz Barbara, Milewski Sławomir, Van Dijck Patrick, Szweda Piotr
Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Str., 80-233 Gdańsk, Poland.
Department of Chemistry, Technology and Biotechnology of Food, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Str., 80-233 Gdańsk, Poland.
Pathogens. 2018 Jun 19;7(2):56. doi: 10.3390/pathogens7020056.
The aim of our work was to check if one of the products of natural origin, namely honey bee propolis, may be an alternative or supplement to currently used antifungal agents. The activity of 50 ethanolic extracts of propolis (EEPs), harvested in Polish apiaries, was tested on a group of 69 clinical isolates of . Most of the EEPs showed satisfactory activity, with minimum fungicidal concentrations (MFC) mainly in the range of 0.08⁻1.25% (/). Eradication of biofilm from polystyrene microtitration plates in 50% (MBEC, Minimum Biofilm Eradication Concentration) required concentrations in the range of 0.04% (/) to more than 1.25% (/). High activity was also observed in eradication of biofilm formed by and on the surfaces of PVC (Polyvinyl Chloride) and silicone catheters. EEPs at subinhibitory concentrations inhibited yeast-to-mycelia morphological transformation of in liquid medium and mycelial growth on solid medium. A synergistic effect was observed for the action of EEP in combination with fluconazole (FLU) and voriconazole (VOR) against . In the presence of EEP at concentrations as low as 0.02%, the MICs of FLU and VOR were 256 to 32 times lower in comparison to those of the drug alone. Evidence for the fungal cell membrane as the most probable target of EEPs are presented.
我们研究的目的是检验一种天然来源的产品，即蜜蜂蜂胶，是否可能成为当前使用的抗真菌剂的替代品或补充剂。对从波兰养蜂场采集的50种蜂胶乙醇提取物（EEPs）的活性进行了测试，受试对象为一组69株临床分离的[具体菌种未给出]。大多数EEPs表现出令人满意的活性，最低杀菌浓度（MFC）主要在0.08⁻1.25%（/）范围内。从聚苯乙烯微量滴定板上根除生物膜（最低生物膜根除浓度，MBEC）所需的浓度在0.04%（/）至超过1.25%（/）范围内。在根除由[具体菌种未给出]在聚氯乙烯（PVC）和硅胶导管表面形成的生物膜方面也观察到了高活性。亚抑制浓度的EEPs抑制了[具体菌种未给出]在液体培养基中的酵母到菌丝体的形态转变以及在固体培养基上的菌丝体生长。观察到EEP与氟康唑（FLU）和伏立康唑（VOR）联合作用对[具体菌种未给出]具有协同效应。在EEP浓度低至0.02%的情况下，与单独使用药物相比，FLU和VOR的最低抑菌浓度（MIC）降低了256至32倍。文中还给出了真菌细胞膜最有可能是EEPs作用靶点的证据。
