Dégi János, Imre Kálmán, Herman Viorel, Bucur Iulia, Radulov Isidora, Petrec Oana-Cătălina, Cristina Romeo Teodor
Faculty of Veterinary Medicine, Banat's University of Agricultural Sciences and Veterinary Medicine Timișoara, Calea Aradului 119, 300645 Timișoara, Romania.
Faculty of Agriculture, Banat's University of Agricultural Sciences and Veterinary Medicine Timișoara, Calea Aradului 119, 300645 Timișoara, Romania.
Antibiotics (Basel). 2021 Nov 16;10(11):1404. doi: 10.3390/antibiotics10111404.
The present study was undertaken to investigate the presence of spp. in the faeces of client-owned cats in urban areas and to evaluate the risk that is posed to public health. Fresh faecal samples were collected directly from the rectums from 53 diarrhoeic and 32 non-diarrhoeic cats. The samples were individually screened for the presence of spp. using standard methods and, in the case of positive findings, the resulting typical colonies were then biochemically confirmed using the VITEK2 automated system. Subsequently, all of the spp. isolates were molecularly tested for the presence of the gene. All of the isolates were serotyped using the slide agglutination technique according to the White-Kauffmann-Le Minor scheme. The phenotypic antimicrobial susceptibility profile of the isolated strains was obtained from the VITEK2 system using specific cards from the Gram-negative bacteria. A total of 16 of the samples (18.82%) tested positive for spp. according to conventional and molecular testing methods. Serotyping of the isolates showed the presence of three serotypes, namely ( = 9; 56.3%), ( = 4; 25%), and ( = 3; 18.8%). All of the tested strains showed strong resistance towards cefazolin, cefepime, ceftazidime, and ceftriaxone. Additionally, resistance (listed in descending order of strength) was observed to trimethoprim/sulfamethoxazole (11/16; 68.8%), ampicillin (10/16; 62.5%), ampicillin/sulbactam (9/16; 56.3%), gentamicin (9/16; 56.3%), nitrofurantoin (8/16; 50.0%), and amikacin (5/16; 31.3%). No resistance was expressed against ciprofloxacin, ertapenem, imipenem, levofloxacin, piperacillin/tazobactam, and tobramycin. The results of this study highlight a substantial public health issue and medical concern, especially in vulnerable people, such as children, the elderly, and immunocompromised individuals.
本研究旨在调查城市地区宠物猫粪便中[具体菌名]的存在情况，并评估其对公众健康构成的风险。直接从53只腹泻猫和32只非腹泻猫的直肠中采集新鲜粪便样本。使用标准方法对样本进行逐个筛查，以检测[具体菌名]的存在，若检测结果为阳性，则使用VITEK2自动化系统对产生的典型菌落进行生化确认。随后，对所有[具体菌名]分离株进行分子检测，以确定是否存在[特定基因]。根据White-Kauffmann-Le Minor方案，使用玻片凝集技术对所有分离株进行血清分型。使用革兰氏阴性菌的特定卡片，从VITEK2系统获得分离菌株的表型抗菌药敏谱。根据传统和分子检测方法，共有16个样本（18.82%）检测出[具体菌名]呈阳性。对[具体菌名]分离株的血清分型显示存在三种血清型，即[血清型1]（n = 9；56.3%）、[血清型2]（n = 4；25%）和[血清型3]（n = 3；18.8%）。所有测试菌株对头孢唑林、头孢吡肟、头孢他啶和头孢曲松均表现出较强耐药性。此外，观察到对甲氧苄啶/磺胺甲恶唑（11/16；68.8%）、氨苄西林（10/16；62.5%）、氨苄西林/舒巴坦（9/16；56.3%）、庆大霉素（9/16；56.3%）、呋喃妥因（8/16；50.0%）和阿米卡星（5/16；31.3%）的耐药性（按耐药强度降序排列）。对环丙沙星、厄他培南、亚胺培南、左氧氟沙星、哌拉西林/他唑巴坦和妥布霉素未表现出耐药性。本研究结果突出了一个重大的公共卫生问题和医学关注点，尤其是在儿童、老年人和免疫功能低下者等弱势群体中。
