Dégi János, Herman Viorel, Radulov Isidora, Morariu Florica, Florea Tiana, Imre Kálmán
Department of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Life Sciences "King Mihai I", 300645 Timisoara, Romania.
Faculty of Agriculture, University of Life Sciences "King Mihai I", 300645 Timisoara, Romania.
Antibiotics (Basel). 2023 Jul 19;12(7):1203. doi: 10.3390/antibiotics12071203.
The number of reptiles owned as pets has risen worldwide. Additionally, urban expansion has resulted in more significant human encroachment and interactions with the habitats of captive reptiles. Between May and October 2022, 48 reptiles from pet shops and 69 from households were sampled in the Timișoara metropolitan area (western Romania). Three different sample types were collected from each reptile: oral cavity, skin, and cloacal swabs. identification was based on ISO 6579-1:2017 (Annex D), a molecular testing method ( gene target), and strains were serotyped in accordance with the Kauffman-White-Le-Minor technique; the antibiotic susceptibility was assessed according to Decision 2013/652. This study showed that 43.28% of the pet reptiles examined from households and pet shops carried spp. All of the strains isolated presented resistance to at least one antibiotic, and 79.32% (23/29) were multi-drug-resistant strains, with the most frequently observed resistances being to gentamicin, nitrofurantion, tobramycin, and trimethoprim-sulfamethoxazole. The findings of the study undertaken by our team reveal that reptile multi-drug-resistant is present. Considering this aspect, the most effective way of preventing multi-drug-resistant infections requires stringent hygiene control in reptile pet shops as well as ensuring proper animal handling once the animals leave the pet shop and are introduced into households.
全球范围内，作为宠物饲养的爬行动物数量有所增加。此外，城市扩张导致人类对圈养爬行动物栖息地的侵占和互动更加显著。2022年5月至10月期间，在蒂米什瓦拉大都市区（罗马尼亚西部）对48只来自宠物店的爬行动物和69只来自家庭的爬行动物进行了采样。从每只爬行动物身上采集了三种不同的样本类型：口腔、皮肤和泄殖腔拭子。鉴定基于ISO 6579-1:2017（附录D），这是一种分子检测方法（基因靶点），菌株按照考夫曼-怀特-勒-米诺技术进行血清分型；根据2013/652号决定评估抗生素敏感性。这项研究表明，从家庭和宠物店检查的宠物爬行动物中有43.28%携带 菌属。所有分离出的菌株对至少一种抗生素呈现耐药性，79.32%（23/29）为多重耐药菌株，最常见的耐药性是对庆大霉素、呋喃妥因、妥布霉素和甲氧苄啶-磺胺甲恶唑。我们团队开展的这项研究结果表明存在爬行动物多重耐药 菌。考虑到这一方面，预防多重耐药 菌感染的最有效方法是在爬行动物宠物店进行严格的卫生控制，并确保动物离开宠物店进入家庭后得到妥善处理。
