Guo Lili, Zhao Yongda
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266000, China.
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Antibiotics (Basel). 2021 Nov 19;10(11):1417. doi: 10.3390/antibiotics10111417.
This study aimed to determine the global prevalence and molecular characterization of CTX-M-producing Typhimurium isolates. A total of 330 (15.2%, 330/21779) -positive . Typhimurium were obtained from the public databases in July 2021. Thirteen variants were found in the 330 members of the group, and (26.4%, 88/330) was the most prevalent. The majority of -positive Typhimurium were obtained from humans (59.7%, 197/330) and animals (31.5%, 104/330). The number of -positive Typhimurium increased annually ( < 0.0001). These isolates were primarily found from China, the United Kingdom, Australia, the USA, and Germany. In addition, these isolates possessed 14 distinct sequence types (ST), and three predominated: ST34 (42.7%, 141/330), ST19 (37.0%, 122/330), and ST313 (10.3%, 34/330). The majority of ST34 Typhimurium isolates were distributed in China and mainly from swine. However, the majority of ST19 were distributed in the United Kingdom and Australia. Analysis of contigs showed that the major type of -carrying plasmid was identified as IncI plasmid (52.9%, 27/51) and IncHI2 plasmid (17.6%, 9/51) in 51 -positive S. Typhimurium isolates. In addition, WGS analysis further revealed that co-existed with nine antibiotic-resistant genes with a detection rate over 50%, conferring resistance to five classes of antimicrobials. The 154 virulence genes were detected among these isolates, of which 107 virulence genes were highly common. This study revealed that China has been severely contaminated by -positive Typhimurium isolates, these isolates possessed numerous ARGs and virulence genes, and highlighted that continued vigilance for -positive Typhimurium in animals and humans is urgently needed.
本研究旨在确定产CTX-M的鼠伤寒沙门氏菌分离株的全球流行情况和分子特征。2021年7月从公共数据库中获得了总共330株（15.2%，330/21779）阳性鼠伤寒沙门氏菌。在该组的330个成员中发现了13个变体，其中[变体名称未给出]（26.4%，88/330）最为普遍。大多数阳性鼠伤寒沙门氏菌分离株来自人类（59.7%，197/330）和动物（31.5%，104/330）。阳性鼠伤寒沙门氏菌的数量逐年增加（P<0.0001）。这些分离株主要来自中国、英国、澳大利亚、美国和德国。此外，这些分离株具有14种不同的序列类型（ST），其中三种占主导：ST34（42.7%，141/330）、ST19（37.0%，122/330）和ST313（10.3%，34/330）。大多数ST34鼠伤寒沙门氏菌分离株分布在中国，主要来自猪。然而，大多数ST19分布在英国和澳大利亚。对重叠群的分析表明，在51株阳性鼠伤寒沙门氏菌分离株中，携带[基因名称未给出]的主要质粒类型被鉴定为IncI质粒（52.9%，27/51）和IncHI2质粒（17.6%，9/51）。此外，全基因组测序分析进一步揭示，[基因名称未给出]与9个耐药基因共存，检测率超过50%，赋予对五类抗菌药物的耐药性。在这些分离株中检测到154个毒力基因，其中107个毒力基因非常常见。本研究表明，中国已受到阳性鼠伤寒沙门氏菌分离株的严重污染，这些分离株拥有大量的耐药基因和毒力基因，并强调迫切需要对动物和人类中的阳性鼠伤寒沙门氏菌持续保持警惕。
